Chapter2CentrifugationEnglish[相容模式].PDFVIP

Chapter 2 Centrifugation: Separation of Organelles and Biomolecules BiochemistryBiochemistry andand MolecularMolecular BiologyBiology (BMB)(BMB) 3.1 Introduction 33.22 BasicBasic PrinciplePrinciple ofof sedimentationsedimentation 3.3 Types, care and safety of centrifuges 33.44 PreparativePreparative centrifugationcentrifugation ((exampleexample onon influenza virus and protein complex) 33.55 AnalyticalAnalytical centrifugationcentrifugation AAnallytiticall BiBiochhemiisttry (AB)(AB) 3.4.3 Ultracentrifugation KoolmanKoolman, ColorColor AtlasAtlas ofof BiochemistryBiochemistry (CAB)(CAB), 2nd2nd editionedition 1 General Steps in Biochemical Separation 2 Separation of Macromolecules (CAB) ChChromattographhy, preciipititatition Electrophoresis, ultracentrifugation 3 Introduction (MBM 3.1) PrinciplesPrinciples ofof centrifugationcentrifugation ((離心離心)) A centrifuge is a device for separating particles from a solutionsolution accordingaccording toto theirtheir sizesize, shapeshape, densitydensity, viscosityviscosity of the medium and rotor speed In a solution, particles whose density is higher thanthan thatthat ofof thethe solventsolvent sinksink (sediment),(sediment), andand particles that are lighter than it float to the top. The greater the difference in density, the fasterfaster theythey movemove. IfIf therethere isis nono differencedifference inin density (isopyknic conditions), the particles hover. To take advantage of even tiny diffdifferences iin ddensitity tto separatte variious particles in a solution, gravity can be replaced with the much more powerful “centrifugal force” provided by a centrifuge.