一氧化氮供体对穿膜肽吸收.pptx

Nitric oxide donors can enhance the intestinal transport and absorption of insulin and [Asu1,7]-eel calcitonin in rats;The characteristics of three NO donors, NOC5 NOC12 SNAP as absorption enhancers for peptide drugs were examined in rats . Insulin and [Asu1,7]-eel calcitonin (ECT) were used as a model drug to investigate the effectiveness of the tested enhancers. Experimental method：a modified Ussing chamber method and an in situ closed loop method. Conclution： NO donors possess excellent effectiveness for the use as absorption enhancers of peptide drugs and they are very effective at lower. ;Essay map;1、Experimental notes-1：;1 Experimental notes-2：;1 Experimental notes-3：;2、Methods-1;2、Methods-2;2、Methods-3;Result-3.1 Effect of NO donors on the permeability of insulin across the intestinal membranes;Fig. 2. Effect of various concentrations of NO donors on the permeability of insulin across the colonic membrane;Result-3.2. Effect of carboxy-PTIO on the absorption enhancing effect of NO donors;Result-3.3. Effects of NO donors on the intestinal absorption of insulin;Result-3.3. Effects of NO donors on the intestinal absorption of insulin;Fig. 5.?Effect of various concentrations of NO donors on the pharmacological availability % (PA%) following administration of insulin (20 IU) to the rat colon.. Dotted line represents the level of insulin PA% without NO donors (control). Keys: (●) NOC5, (▲) NOC12 and (■) SNAP;Fig. 6.?Effect of 10 mM carboxy-PTIO (c-PTIO) on the pharmacological availability % (PA%) of insulin after administration to the rat colon with NO donors (5 mM)., significantly different compared with the control, (n.s.) not significantly different compared with the control.;Result-3.4. Stability of insulin in the intestinal mucosal homogenates in the presence of NO donors;Result-3.4. Stability of insulin in the intestinal mucosal homogenates in the presence of NO donors;Result-3.5. Effects of NO donors on the intestinal absorption of ECT;Result-3.5. Ef