可用-Clinical study of a urinary competitve ELISA for neural thread protein in Alzheimer disease..pdfVIP

Michael Munzar, MD ,* Clinical St udy of a Urinary Compet itve † Suzanna Levy, PhD , ELISA for Neural Thread Protein in ‡ Robert Rush, PhD , and Paul Averback, MD* Alzheimer Disease From * Nymox Pharmaceutical Corporation, Montreal, Canada, †Mount Sinai School of Medicine, New York, New York, and ‡ Bio Reference Laboratories Inc., Elmwood Park, New Jersey. n AD7C-NTP (neural thread protein) is Alzheimer disease (AD) is the most common cause of dementia in the a 41-kD brain protein that is selec- Western hemisphere. The disease is characterized pathologically by tively elevated in Alzheimer disease prominent atrophy of corticolimbic structures with neuronal loss, neu- (AD). AD7C-NTP is associated with the ro brillary tangle (NFT) formation, aberrant proliferation of neurites, se- pathologic changes of AD, and overex- nile plaques, and amyloid deposition in the brain . More than 90% of pression of the AD7C-NTP gene is asso- AD is sporadic. The reported accuracy of antemortem clinical diagnosis ciated with cell death similar to that of AD is quite variable, and a reliable biochemical marker is needed to found in the AD brain. A newly devel- help in the accurate and early diagnosis of AD (Molsa et al, 1985; Rocca oped competitive ELISA (enzyme-linked et al, 1986; Burns et al, 1990; Risse et al, 1990; Gilleard et al, 1992; immunosorbent assay) was tested in Mendez et al, 1992; Corey-Bloom et al, 1995; Fleming et al, 1995; Bow- urine samples from patients with AD, ler et al, 1998; Lannfelt, 1998; Sandson and Price, 1999; Galton et al, patients with non-AD dementia, and 2000; Diaz, 2001). There is an average delay of nearly 3 years from healthy normal individuals. Mean assay when init