微免试验考察(国外英文资料).docVIP

微免实验考核 Basic operation a gram staining method Operation method 1. The initial dye drops 2 ~ 3 drops to smear, dyeing time 30 seconds Dye the alkaline iodine drop 2 ~ 3 drops to smear for 30 seconds Remove the acetone alcohol from 2 to 3 drops to the smear for 5 to 10 seconds Redye the diluted red drops 2 ~ 3 drops to smear for 5 seconds To answer the relevant question 1 staining positive bacterium is a purple negative bacterium is red The cause of false positives or false negatives is that the discoloration time is too short or too long The significance of dyeing is to classify bacteria as the guiding drug Basic operation 2 anti-acid staining Operation method First dye and accelerant drop 2 ~ 3 drops to smear for 60 seconds Remove the hydrochloric acid from alcohol and remove it until it is colorless 3 redye the alkaline blue drops 2 ~ 3 drops to smear for 60 seconds Answer questions 1 staining positive bacterium is a red negative bacterium is blue The staining significance identifies tuberculosis bacillus Basic operation 3 ink dyeing method Operation method Take a drop of ink and drop it on a slide 2 take the bacteria and get the bacteria The mixed inoculation ring has a bacterial part and contact with the ink several times Slide the slide first and press the other side of the slide Answer questions The capsule was visible in a dye The significance of dyeing is to identify the new cryptococcus Basic operation 4 solid bacteria smear Operation method Take 1 ~ 2 rings of saline and drip onto the slide 2 take the bacteria and get the bacteria Spread the film evenly and spread out from the inside out Dry and dry, dry or dry Fixed in the flame of an alcohol lamp three times fast Answer questions A fixed effect of sterilization adhesive Basic operation 5 sputum bacteria smear Operation method Pick up the sputum with a cotton swab The smear is spread out from the inside out Dry, dry and dry Fixed in the flame of an alcohol lamp three times fast Answer questions A fixed ef