牦牛PLIN2基因的克隆及其在卵巢不同发情阶段的表达.pdfVIP

2017 44 3 782789 China A nim al H usbandry Vete r inary M e d ic ine doi cnki j P L I N2 PL I N GenBank RTPCR PL I N RTPCR PL I N PCR PL I N bp GenBank PL I N PLIN PCR PLIN PL I N PL I N PCR Q A Cloning of PLIN2 Gene of Yak and Its Expression in Ovary during Different Estrus Periods CAI Wen i CHEN Tong SHI Xian CHEN Weiming HU Jiaia y j XIONG Xianrong LAN Daoliang LI Jian College of L if e Science and Technology Southwest University f or N ationalities Chengdu China Institute o Qin haiTibetan Plateau Southwest Universit or N ationalities Chen du China f g y f g Abstract T he purpose of this study w as to research the sequence characteristics and the ex pres sion of PL I N ene and to analyze the relationship betw een the ex pression and the ovaries in g diff erent estrus periods According to the gene sequence of bovine in GenBank the primer w as desig ned T he PL I N ene of y ak w as cloned by RT PCR and the ex pression quantity of the g PL I N ene in diff erent tissues of y ak w as detected by the semiquantitative RT PCR M ean g w hile the diff erential ex pression of the PL I N ene in diff erent ovarian estrus periods of y ak w as g detected by qua