下载感受态细胞说明书-GeneCopoeia.PDFVIP

TMTM GeneCopoeia GeneCopoeia Expressway to DiscoveryExpressway to Discovery Protocol • Chemically Competent Cells • Catalog Nos. STK200-10/20, STK300-10/20 Efficient transformation, stable inserts and high-quality plasmid DNA Description GeneCopoeia chemically competent cells provide high-efficiency transformation and are suitable for many standard cloning applications. The cells enable fast screening with blue/white selection, ensure high-quality plasmid preparations and contain recA1 endA 1 mutations to increase insert stability. Available in single tube format, GeneCopoeia competent cells deliver a reliable platform for producing plasmid DNA . GeneCopoeia chemically competent cells GCI-5α and GCI-L3 are comparable to DH5α™ and Stbl3™ respectively. Strain GCI-5α GCI-L3 Applications Transformation of standard (non- Transformation of lentiviral plasmid lentiviral) plasmid constructs constructs Catalog numbers STK200-10 (10 tubes) STK300-10 (10 tubes) STK200-20 (20 tubes) STK300-20 (20 tubes) Transformation efficiency 1 - 3 x 109 8 cfu/μg pUC19 DNA 1×10 cfu/µg pUC19 Size 100 µl/tube 100 µl/tube Shipping and storage Ship on dry ice and store Ship on dry ice and store conditions immediately at –80 °C immediately at –80 °C Storage of plasmid samples Plasmid DNA samples in TE buffer (pH 8.0) should be stored at 4°C. Transformation protocol