兔眼蓝莓组织培养过程中褐化与生根问题的探讨.PDF

安徽农业大学学报, 2012, 39(5): 777-782 Journal of Anhui Agricultural University 网络出版时间：2012-8-27 12:04:22 [URL] /kcms/detail/34.1162.S1204.029.html 兔眼蓝莓组织培养过程中褐化与生根问题的探讨 郑理乔，黄成林，刘 华，梅 莹，傅松玲* (安徽农业大学林学与园林学院，合肥 230036) 摘 要：以兔眼蓝莓茎尖和茎段形成的愈伤组织和长期继代培养的试管苗为材料，接种于改良的 WPM 和 1/2 WPM 的培养基中，研究活性炭与 Vc 液对兔眼蓝莓外植体和愈伤组织形成中褐化的影响以及外源体、活性炭对无 根苗瓶内生根的影响。结果表明，当培养基中加入 Vc 液并含 100 ～200 mg·L-1 的活性炭时，能有效防止外植体褐 化的发生；当培养基中加入 Vc 液并含 200 ～500 mg·L-1 活性碳时，可有效防止愈伤组织褐化发生。蓝莓继代培养 试管苗在 1/2 WPM 的培养基中添加 0.2 mg·L-1 IBA ，并含 100 ～200 mg·L-1 活性炭时生根情况最为理想，经过 7 d 暗培养，30 d 后生根率高达 87.8%。 关键词：兔眼蓝莓；组织培养；褐化；瓶内生根 中图分类号：S723.132.6 文献标识码：A 文章编号：1672−352X (2012)05−0777−06 Discussion on browning and rooting in tissue culture propagation of Rabbit-eyes blueberry ZHENG Li-qiao, HUANG Cheng-lin, LIU Hua, MEI Ying, FU Song-ling (School of Forestry and Landscape Architecture, Anhui Agricultural of University, Hefei 230036) Abstract: In this paper, callus and long-term trained tube seedlings induced from stem tip and stem section of Rabbit-eyes blueberry were inoculated to the modified WPM and 1/2 WPM mediums to study the effects of actived carbon and Vc on browning reaction. The effects of exogenous substance, activated carbon on the plant roots were also studied. The results showed that the explant browning could be controlled if the medium contained -1 Vc liquid and activated carbon of 100-200 mg·L . The callus browning could be effectively prevented in the me- -1 dium contained Vc liquid and activated carbon of 200-500 mg·L . The suitable condition for blueberry training -1 -1 was the modified 1/2