千家寨不同海拔野生茶树的+EST-SSR+遗传多样性研究.pdfVIP

茶叶科学 20 15，35 （4 ）：347~353 Journal of Tea Science 投稿平台： 千家寨不同海拔野生茶树的EST-SSR 遗传多样性研究 黄晓霞，唐探，姜永雷，冯程程，程小毛* 西南林业大学园林学院，云南 昆明 650224 摘要：利用 5 对 EST-SSR 引物对千家寨内 7 个海拔梯度的野生茶树居群进行遗传多样性和遗传结构的研究。 在物种水平上，Shannon 信息指数（I ）和Nei 基因多样性（He ）分别为1.33 和 0.66 ，表现出很高的遗传多样 性；千家寨不同海拔梯度上野生茶树居群的遗传多样性有差异，且随着海拔梯度的递增，居群的遗传多样性 呈现出低—高—低的分布，并在海拔 2 100 m 处达到最大值；野生茶树居群间的基因流（Nm ）为 1.84，群体 分化系数（Fst ）为0.12 ，且基于AMOVA 软件分析结果显示有 16.32%的变异发生在居群间，表明野生茶树群 体间属于中度分化，且大部分变异存在于居群内。野生茶树本身的遗传特性和不同海拔居群所处生境的异质 性是其现有遗传格局的主要原因。 关键词：野生茶树；海拔梯度；遗传多样性；遗传分化；EST-SSR 中图分类号：S571.1 ；S339 文献标识码：A 文章编号：1000-369X(2015)04-347-07 Genetic Diversity of Wild Tea Plant in Different Altitude in Qianjiazhai * HUANG Xiaoxia , TANG Tan, JIANG Yonglei, FENG Chengcheng, CHENG Xiaomao Faculty of Landscape Architecture , Southwest Forestry University, Kunming 650224, China Abstract: 5 pairs of EST-SSR markers derived by the authors were used to study genetic diversity and genetic structure of 7 wild tea populations at different altitudes in Qianjiazhai. At the species level, Shannon information index (I) and Nei ’s gene diversity (He) were 1.33 and 0.66, respectively, indicating this species has high genetic diversity at species level. Along altitudinal gradients, the genetic diversity among population of wild tea was different and showed low-high-low distribution. The genetic diversity of population at altitude 2 100 m was much higher than others populations. AMOVA analysis showed that only 16.32% of the total genetic