大孔吸附树脂分离纯化肉苁蓉中苯乙醇苷工艺研究.PDF

大孔吸附树脂分离纯化肉苁蓉中苯乙醇苷工艺研究 顾家珲 1 ，余晓晖 1, 2 ，吴蓉 1 ，赵信科 3 ，赵磊 1, 2*(1.甘肃中医学院药学系，兰州 730000 ；2.甘肃省高校中藏药化学与质量研究 省级重点实验室，兰州 730000 ；3.兰州铝厂职工医院，兰州 730060) 摘要：目的 筛选富集肉苁蓉中苯乙醇苷的最佳树脂和工艺条件，使分离纯化工艺达到最优化。方法 选择 7 种大孔吸 附树脂(AB-8、DM301 、DM130 、D1400 、DA-201 、HPD-100 、D-101-I) ，通过树脂对苯乙醇苷的静态吸附和解析能力， 筛选出最佳树脂，再通过树脂的静态特性和动态特性的筛选优化工艺。结果 DA-201 的吸附解析最佳条件为：上样流速 1 ，洗脱剂为 90%的乙醇。按最佳工艺苯乙醇苷的纯度由 25.1%上升为 51.2%。结论 该法简便、可行，为 为 1.5 mL·min 肉苁蓉中苯乙醇苷的纯化提供了参考。 关键词：肉苁蓉；苯乙醇苷；大孔吸附树脂；纯化 中图分类号：R284.2 文献标志码：B 文章编号：1007-7693(2011)11-1006-04 Optimization of the Isolation and Purification Technology of Phenylethanoid Glycosides from Cistanche Tubulosa by Macroporous Adsorption Resin GU Jiahui1 1, 2 1 3 1, 2* , YU Xiaohui , WU Rong , ZHAO Xinke , ZHAO Lei (1.Department of Pharmacy, Gansu College of TCM, Lanzhou 730000, China; 2.Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province, Lanzhou 730000, China; 3.Lanzhou Aluminium Plant Staff Hospital, Lanzhou 730060, China) ABSTRACT: OBJECTIVE To investigate the optimal macroporous adsorption resin and process to separate phenylethanoid glycosides from Cistanche tubulosa. METHODS Seven types of macroporous adsorption resin were evaluated for separating efficiency by measuring the adsorption ratio, eluting ratio of phenylethanoid glycosides. RESULTS The optimal technologies of DA-201 macroprous dosorption resin were as follows: the simple flow was 1.5 mL·min1 , the column was washed by 95% ethanol. After enrichment and purification, the product purity of phenylethanoid glycosides was up to 51.2%. CONCLUSION This method is simple, feasible and can provide useful reference for the isolation and purification of phenylethanoid glycoside