[2017年整理]分子生物学试卷(英文).docVIP

西南大学 《 Molecular Biology 》课程 2011～2008学期 期 考试时间 120分钟 考核方式 学生类别 人数 98 适用专业或科类 生物技术 年级 05 级 题号 合计 得分 签名 阅卷须知：阅卷用红色墨水笔书写，得分用阿拉伯数字写在每小题题号前，用正分表示，不得分则在题号前写0；大题得分登录在对应的分数框内；统一命题的课程应集体阅卷，流水作业；阅卷后要进行复核，发现漏评、漏记或总分统计错误应及时更正；对评定分数或统分记录进行修改时，修改人必须签名。 I Define the meaning of these following terms (30 points, 3 pts per term) melting temperature (Tm) C value paradox exon semi-conservation replication cDNA library gel shift assay promoter open reading frame (ORF) gene family expressed sequence tag (EST) II Fill in blank (10 points, 1 pts per blank) 1. The simplest cloning vector at least contains , and ori elements. 2. The diagram below shows two RNA molecules. Molecule I is processed to give molecule II. Answer the following questions about these two molecules: Where does this conversion happen? ____________________________ What type of RNA molecule is molecule I? _________________________ What type of RNA molecule is molecule II? _________________________ Name the regions/structures indicated by the arrows: A = _________________ B = _________________ C = _________________ D = _________________ E = _________________ III Select the right answer (20 points, 2 pts per question; Select the best answer for each question.) 1. The bond between a phosphate group and the ribose sugar groups in RNA is called which of the following? ( ) (A) Phosphodiester bond (B) Glycosidic bond (C) Peptide bond (D) Amide bond 2. Which enzyme does not play a role in DNA replication? ( ) (A) DNA polymerase (B) Helicase (C) Single-stranded binding protein (D) Guanyl transferase 3. Splicing is the process that does which of the following? ( ) (A) Remove introns and conserve exons (B) Remove exons and conserve introns (C) Remove mutated regions of primary transcript RNA (D) Add multiple adenosine bases to the end of a primary RNA transcript 4. The ideal spacing