HSV-2gB(1-G-10)sc-52425.PDFVIP

SAN TA C RUZ BI OTEC HNOL OG Y, INC . HSV-2 gB (1-G-10): sc-52425 BACKGROUND APPLICATIONS Membrane fusion is crucial for the entry, spread and formation of enveloped HSV-2 gB (1-G-10) is recommended for detection of HSV-2 gB of Herpes viruses, such as herpes simplex virus, and is mediated by envelope glycopro - Simplex Virus 1 origin by Western Blotting (starting dilution 1:200, dilution teins. Two serotypes of the herpes simplex virus, type 1 (HSV-1, oral) and range 1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total type 2 (HSV-2, genital), have been shown to encode at least ten glycoproteins, protein (1 ml of cell lysate)] and immunofluorescence (starting dilution 1:50, four of which are necessary and sufficient to facilitate fusion. These four dilution range 1:50-1:500). glycoproteins include glycoprotein B (gB), glycoprotein D (gD), glycoprotein H Molecular Weight of HSV-2 gB: 113 kDa. (gH) and glycoprotein L (gL). The fusion event is dependent upon the expres - sion of a gD receptor on target cell membranes and does not require the SELECT PRODUCT CITATIONS presence of cell-surface glycosaminoglycans. Glycoprotein B is essential for viral growth, as gB-free virions are able to bind cells but not to synthesize 1. Cheshenko, N., et al. 2013. HSV activates Akt to trigger calcium release virus-specific proteins upon infection. HSV-1 and HSV-2 gB exist as a homo- and promote viral entry: novel candidate target for treatment and sup - dimer which may be linked by disulfide bonds. HSV-1 gB is a 904 amino acid pression. FASEB J. 27: 2584-2599. protein with an extracellular domain consisting of amino acids 31-730 and a 2. Liu, Y., et al. 2015. Tetherin restricts HSV-2 release and is counteracted by cytoplasmic domain consisting of