水稻内生菌的分离纯化与鉴定03较完整.docVIP

谢香凤 水稻内生菌的分离及形态观察 玉林师范学院本科生毕业论文 玉林师范学院本科生毕业论文 水稻内生菌的分离及形态观察 Separate and morphologic observation of endophyte in rice 院 系生命科学与技术学院专 业生物技术学 生 班 级生技08班姓 名谢 香 凤学 号200806403136指导教师单位生命科学与技术学院指导教师姓名林谦指导教师职称副教授(博士) 水稻内生菌的分离及形态观察 生技08班 谢 香 凤 指导教师 林谦 摘要 水稻是世界上最重要的粮食作物之一，是亿万人口的基本营养来源。随着世界人口的日益增多和耕地面积的急剧减少，水稻增长措施的研究已经成为当今农业科学研究的重要课题。经平板培养法分离出水稻内生真菌和细菌，再采用平板落计数法对水稻内生菌的根、茎、叶长出的内生菌的数量测定，并分别取出它们的平均值，再用F检验根茎叶有无显著性差异。然后用显微镜观察水稻的内生菌的形态。水稻细菌的根茎叶生长趋势表明，水稻内生细菌在植株器官中的分布以根部数量最多，其次是叶，茎最少；而水稻真菌的根茎叶生长趋势表明：水稻内生真菌在植株器官中以根部数量最多。但分布明显比内生细菌少。通过对内生菌的分离及形态观察，并在显微镜观察可以鉴别水稻内生菌属于哪个属，研究水稻的内生菌的分布情况，可以更有效的利用植物内生菌为我们谋福利。 关键词: 水稻、内生细菌、内生真菌、分离、形态观察 Separate and morphologic observation of endophyte in rice Biotechnology Class 08 Xie Xiang-feng Supervisor Lin-qian Abstract Rice is one of the most important grain crop in the world. It is the primary nutrition source of billionaire population. Along with world population increases every day and arable areas reduce sharply, nowadays, the study of rice increase have became an important topic in agricultural sciences research. Through plating method separates endophyte fungi and bacteria of rice, than adopt enumeration by platecount method to determine or measure the quantity of rice endophytic roots, stems and leaves. Their average value were separate derived. Than we can use F-test to examine whether roots, stems and leaves have significant difference or not. At last, electron microscope observe morphology of this endophyte fungi and bacteria in rice. The bacterial growth tendency of stem, root and leaf in rice indications that endophytic bacteria in rice plants with roots in the distribution of organs account for the greatest number, followed by leaves, stems at least. However, the growth tendency of stem , root and leaf in rice endophytic fungi indications that endophytic fungi in rice plants with roots in the distribution of organs account for the greatest number. But the distribut