Jasmonic acid prevents the accumulation of cyclin B1 1 and CDK-Bin synchronized tobacco BY-2 cells.pdfVIP

Jasmonic acid prevents the accumulation of cyclin B1 1 and CDK-Bin synchronized tobacco BY-2 cells.pdf

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Jasmonic acid prevents the accumulation of cyclin B1 1 and CDK-Bin synchronized tobacco BY-2 cells

FEBS Letters 572 (2004) 118–122 FEBS 28664Jasmonic acid prevents the accumulation of cyclin B1;1 and CDK-B in synchronized tobacco BY-2 cells Agnieszka Swiazteka, Abdelkrim Azmia, Hilde Stalsb, Dirk Inzeb, Harry Van Onckelena,* aLaboratory of Plant Biochemistry and Physiology, Department of Biology, University of Antwerp (UA), Universiteitsplein 1, B-2610 Antwerp, Belgium bDepartment of Plant Systems Biology, VIB-Ghent University, Technologie Park 927, B-9052 Ghent, Belgium Received 2 June 2004; revised 7 July 2004; accepted 9 July 2004 Available online 21 July 2004 Edited by Ulf-Ingo Fl€uggeAbstract Jasmonic acid (JA) plays a crucial role in plant fertility and defense responses. It exerts an inhibitory effect on plant growth when applied exogenously. This effect seems to be somehow related to a negative regulation of cell cycle progres- sion in the meristematic tissues. In this report, we focus on the molecular events that occur during JA-induced G2 arrest. We demonstrate that JA prevents the accumulation of B-type cyclin- dependent kinases and the expression of cyclin B1;1, which are both essential for the initiation of mitosis. This feature suggests the existence of an early G2 checkpoint that is affected by JA.  2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. Keywords: Jasmonic acid; Cell cycle; Cyclin B1;1; Roscovitine; G2/M transition1. Introduction Cyclin-dependent kinases (CDKs) play a central regulatory role during cell division in all eukaryotic cells [1]. Their activity is strictly controlled at the time of the G1/S and G2/M tran- sitions of the cell cycle by means of phosphorylation and in- teractions with regulatory subunits [2]. One class of those subunits, called cyclins, determines the substrate specificity and subcellular localization of the kinase [3]. Cyclins are expressed at specific stages of the cell cycle and are rapidly degraded. There are three major types of cyclins in plants. D-type cyclin

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