Inhibition of lung cancer cell proliferation mediated by human mesenchymal stem cells.pdf

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Inhibition of lung cancer cell proliferation mediated by human mesenchymal stem cells.pdf

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Inhibition of lung cancer cell proliferation mediated by human mesenchymal stem cells

Original Article Inhibition of lung cancer cell proliferation mediated by human mesenchymal stem cells Lin Li1, Hui Tian1*, Zhitao Chen2, Weiming Yue1, Shuhai Li1, and Wenjun Li1 1Department of Thoracic Surgery, Qilu Hospital, Shandong University, Jinan 250012, China 2Department of Thoracic Surgery, Jinan Central Hospital, Shandong University, Jinan 250012, China *Correspondence address. Tel: t86-531 Fax: t86-531 E-mail: tianhuiy@ Human mesenchymal stem cells (hMSCs) are mostly studied for their potential clinical use. Recently, much attention in the field of cancer research has been paid to hMSCs. In this study, we investigated the influence of hMSCs on the proliferation of lung cancer cell lines SK- MES-1 and A549 in vitro and in vivo by using a co-culture system and the hMSCs-conditioned medium. Our results demonstrated that hMSCs could inhibit the proliferation of SK-MES-1 and A549 cells, and induce the apoptosis of tumor cells in vitro via some soluble factors. Animal study showed that these soluble factors from hMSCs could sup- press tumorigenesis and tumor angiogenesis by treating preliminarily tumor cells with the hMSCs-conditioned medium. The downregulated expression of vascular endo- thelial growth factor in tumor cells might be the mechan- ism of interference in tumor angiogenesis, which was verified by western blot analysis and immunohistochemis- try assay. Taken together, our results suggested that the hMSCs could inhibit tumor cell growth by secreting some soluble factors. Keywords mesenchymal stem cell; lung cancer; proliferation; vascular endothelial growth factor Received: September 28, 2010 Accepted: November 12, 2010 Introduction Mesenchymal stem cells (MSCs), a rare non-hematopoietic population in the adult bone marrow (BM), could self- renew and differentiate into mesodermal cell lineages, and support hematopoiesis [1,2]. In addition, there was some evidence to show that MSCs have a potent immunosup- pressive ability by inhib