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Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells.pdf

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Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells

BioMed CentralMolecular Cancer ssOpen AcceResearch Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells Dong-Cheol Seo1, Ji-Min Sung1, Hee-Jung Cho1, Hee Yi1, Kun-Ho Seo2, In-Soo Choi3, Dong-Ku Kim4, Jin-Suk Kim1, Abd El-Aty AM1 and Ho-Chul Shin*1 Address: 1Department of Veterinary Pharmacology and Toxicology College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Republic of Korea, 2Department of Public Health, College of Veterinary Medicine, Konkuk University, 1 Hwayang- dong, Kwangjin-gu, Seoul 143-701, Republic of Korea, 3Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Republic of Korea and 4Cell and Gene Therapy Research Institute, Pochon CHA University, CHA General Hospital, Seoul 135-081, Republic of Korea Email: Dong-Cheol Seo - sdc97@; Ji-Min Sung - lava0331@; Hee-Jung Cho - hjcho@konkuk.ac.kr; Hee Yi - yahhee@; Kun-Ho Seo - bracstu3@konkuk.ac.kr; In-Soo Choi - ischoi@konkuk.ac.kr; Dong- Ku Kim - dokukim@; Jin-Suk Kim - jskim@konkuk.ac.kr; Abd El-Aty AM - abdelaty44@; Ho- Chul Shin* - hshin@konkuk.ac.kr * Corresponding author Abstract Background: The studies on cancer-stem-cells (CSCs) have attracted so much attention in recent years as possible therapeutic implications. This study was carried out to investigate the gene expression profile of CSCs in human lung adenocarcinoma A549 cells. Results: We isolated CSCs from A549 cell line of which side population (SP) phenotype revealed several stem cell properties. After staining the cell line with Hoechst 33342 dye, the SP and non- side population (non-SP) cells were sorted using flow cytometric analysis. The mRNA expression profiles were measured using an Affymetrix GeneChip? oligonucleotide array. Among the sixty one differentially expressed genes, the twelve genes inclusive three poor prognostic genes; Aldo-keto reductase family 1, member C1/C2 (