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PosttranslationalModificationofMaizeChloroplastPyruvate
Posttranslational Modification of Maize Chloroplast Pyruvate Orthophosphate Dikinase Reveals the Precise Regulatory Mechanism of Its Enzymatic Activity by Yi-Bo Chen, Tian-Cong Lu, Hong-Xia Wang, Jie Shen, Tian-Tian Bu, Qing Chao, Zhi-Fang Gao, Xin-Guang Zhu, Yue-Feng Wang, and Bai-Chen Wang Plantphysiol Volume 165(2):534-549 June 2, 2014 ?2014 by American Society of Plant Biologists Light intensity regulated the reversible phosphorylation of maize chloroplast PPDK at Thr-527. Yi-Bo Chen et al. Plant Physiol. 2014;165:534-549 ?2014 by American Society of Plant Biologists Determination of the N-terminal amino acid sequences of different PPDK isoforms using Edman degradation and UHPLC-MS/MS. Yi-Bo Chen et al. Plant Physiol. 2014;165:534-549 ?2014 by American Society of Plant Biologists Locations of the four phosphorylation residues in the primary sequence and the three-dimensional structure of C4PPDK. Yi-Bo Chen et al. Plant Physiol. 2014;165:534-549 ?2014 by American Society of Plant Biologists Site-directed mutagenesis of maize C4PPDK reveals that Ser-528 is another target of PDRP. After incubation with PDRP, the phosphorylation status of wild-type maize C4PPDK and point mutants was identified using specific antibodies against Thr-527 (A) and Ser... Yi-Bo Chen et al. Plant Physiol. 2014;165:534-549 ?2014 by American Society of Plant Biologists The two H-bonds between Ser-528 and Gly-525 play an essential role in the regulation of phosphorylation at Thr-527 by PDRP. A, The atomic-level environment of H-bond formation between Gly-525 and Ser-528. Yi-Bo Chen et al. Plant Physiol. 2014;165:534-549 ?2014 by American Society of Plant Biologists Mechanism of convergent evolution of H-bond formation in the PEP-utilizer domain of C4PPDK and PEPS. A, Alignment of primary sequences of C4PPDK and its homolog PEPS among different species. Yi-Bo Chen et al. Plant Physiol. 2014;165:534-549 ?2014 by American Society of Plant Biologists Light intensity regulated the reversible
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