乳腺特异表达载体缺失片段的鉴定与修复.pdf

554m中国动物遗传育种研究进展 TwoKindof Plasmld pGI-ImlMyogenic-expression for RatGrowth Enhancing YalP XialLi MengQingyon91Zhang Gong Nill91 (1．Sc／eM．e college，State协laboratoryforogrobiotechnology，ChinaAgricaltutnl (2．Food college，ChinaAgriculturalUniversity，Beijing，100094) of of Abstract：Twokins ectopicexpressionpiggrowthhormone—releasing constructeda A53f-H6·GHRH(ppG-H6)andppG—A53f-H4-GHRH(pr,G-rt4)，werebyusingmyogenicexpression vectorwhichhasa-actin elements．Aftertreatedthetwo inmuscle．therathasa expr∽ion by plasmids greatgrowth thanthecontr01．Thetreated showedanlolteffective withthecontrol gain ppG—H6 group result．Cempafing group，at the33rd Was onthis dayafterinjected，theweight鲥nofppoH6group signifieant(200．579vsl85．859)．Based wasa for research，itgood way enhancinganimal舻owthbyinjectingnryogenicexpressionplasmid． Keywords：GHRH，Myogenicexpressiort，Enhancinggrowth，Plasnfid，Genetherapy 乳腺特异表达载体缺失片段的鉴定与修复① 赵志辉1，3，。王毅彰4赵要风2樊宝良1， LennartHanmrstrom2 李宁1，。 (1．中国农业大学农业生物技术国家重点实验室，北京，100094) lnstltule，Karolinska (2．Karolinska F79，SEl4186，Sweden) UniversityHospital，Huddinge (3．吉林大学农学部，长春，130062) (4．河南省安阳师范学院地理系，安阳，455002) 摘要：通过对pBcl中的乳腺特异表达启动子成分进行分析，鉴定了位于p_酪蛋白启动子上游两 个EcoR 有启动子成分发生了位置改变。通过与山羊B．酪蛋白启动子序列进行比较，设计并合成了缺失片段，经 一系列