纳米抗体及其在诊断检测中的进展概述.pdf

孔庆明 等/纳米抗体及其在诊断检测中的研究进展 1351 Chinese Journal of Biotechnology /cjbcn September 25, 2014, 30(9): 1351?1361 DOI: 10.13345/j.cjb.130585 ?2014 Chin J Biotech, All rights reserved 310013 , , , . . , 2014, 30(9): 1351?1361. Kong QM, Yao YB, Chen R, et al. Progress in nanobody and its application in diagnosis. Chin J Biotech, 2014, 30(9): 1351?1361. : (NanobodyNb) Nb Nb Nb Nb Nb Nb 3 Nb Nb Nb : Progress in nanobody and its application in diagnosis Qingming Kong, Yabo Yao, Rui Chen, and Shaohong Lu Institute of Parasite Disease, Zhejiang Academy of Medical Sciences, Hangzhou 310013, Zhejiang, China Abstract: Nanobodies are derived from the variable domain of the heavy-chain antibodies (HCAbs) that occur naturally in the serum of Camelidae. They are the smallest antibody fragments capable to bind antigens. With the characteristics of their increased solubility, increased domain stabilities, nanomolar affinities, easy crossing the blood-brain barrier, easy generation, engineering, optimization and tailoring, easy humanization, nanobodies have extensive application prospects in diagnosis and detection. Although nanobody has demonstrated tremendous success, a number of practical challenges limit its broader applications in disease diagnosis and detection, including construction of a phage library and selection of nanobody fragments with high affinity and immunogold labeling technique. Here, we review several recent findings on the Received: November 19, 2013; Accepted: January 27, 2014 Supported by: Intenational ST Cooperation Program of China (No.