蜂针对佐剂性大鼠关节炎滑膜细胞因子基因表达的影响.docVIP

蜂针对佐剂性大鼠关节炎滑膜细胞因子基因表达的影响 杨顺益 林军 邓金峰 冯淑兰 李万瑶 张宏 刘金芝 (广州中医药大学，39蜂疗网，广州510407) 内容提要 目的:检测IL-1B、IL-6在类风湿性关节炎(RA)的表达，探讨蜂针治疗RA的作用机理。方法:将40只大鼠随机分为四组，采用PCR微板核酸杂交-ELISA方法检测滑膜IL-1B、IL-6的mRNA表达水平。结果:蜂针组和激素组IL-1B、IL-6的mRNA表达水平无显著性差异，而与模型组比较有显著性差异。结论:RA早期IL-1B、IL-6的mRNA表达水平是上调的，蜂针治疗后可以抑制其转录和表达，有利于RA病情的缓解。 关键词 蜂毒疗法 类风湿 IL-1BmRNA IL-6mRNA 基因表达 EffectofAcupoint-apitherapyonCytokineGeneExpressionofSynovialTissuesinAdjuvantArthritisRat sYANGShunyi，LINJun，DENGJinfeng，FENGShulan，LIWanyao，ZHANGHong，LIUJinzhi(GuangzhouUniversityofTCM，Guangzhou，510407)Objective:Tostudytheeffectofacupoint-apitherapyontheexpressionofIL-1BmRNAandIL-6mRNAandtoanalyzeitsmechanismsintreatmentofrheumatoidarthritis(RA).Methods:Atotalof40ratswererandomlyandevenlydividedintonormalcontrolgroup，modelgroup，hormone(hydrocortisone)groupandacupoint-apitherapygroup.RAmodelwasestablishedbyinjectingFreund.sadjuvant(0.05mL)intotherighthind-paw.Forratsofhormonegroup，intraperitonealinjectionofhydrocortisone(10mg/2mL)wasperformed.Inacupoint-apitherapygroup，/Shenshu0(BL23)，/Zusanli0(ST36)，/Mingmen0(GV4)and/Dazhui0(GV14)werestimulatedwithbee-needleprickingmethod.IL-1BmRNAandIL-6mRNAexpressionofsynovialtissues(rightkneejointcapsule)wasdetectedusingPCRmicroplatesandwichhybridizationtechnique.Results:Incomparisonwithnormalcontrolgroup，IL-1BmRNAandIL-6mRNAexpressionofsynovialtissuesincreasedconsiderablyinmodelgroup(P0.01).Therewasnostatisticaldifferencebetweenacupoint-apitherapygroupandhormonegroupinIL-1BmRNAandIL-6mRNAexpression，buttheexpressionsinthesetwogroupsweresignificantlyweakerthanthoseofmodelgroup.Conclusion:Acupoint-apitherapycouldinduceupregulationofIL-1BmRNAandIL-6mRNAexpressioninRArats，whichmayberesponsibleforapitherapygeneratedreliefofsymptomsandsignsofRA.KeyWordsApitherapyRheumatoidarthritisIL-1BmRNAIL-6mRNAGeneexpression?SSC(3mol/LNaCl，0.3mol/L柠檬酸三钠)、硫酸葡聚糖(PEG)、去离子甲酰胺、十二烷基硫酸钠(SDS)等;杂交洗液:1?SSC;封闭剂:1%Ficoll400，0.5%HS-DNA、0.5%聚乙烯吡咯烷酮(PVP)、1%BSA、脱脂奶粉、甘氨酸。上述溶液均由第一军医大学生物医学诊断研究中心提供。 1.5引物与探针采用 Primer软件设计引物，选用IL