【2017年整理】D NA倍体分析与癌胚抗原检测对良、恶性腹水鉴别诊断.docVIP

世界华人消化杂志 2009年3月8日; 17(7): 724-728 ISSN 1009-3079 CN 14-1260/R 临床经验 CLINICAL PRACTICE D NA倍体分析与癌胚抗原检测对良、恶性腹水鉴别诊断的意义 张爱军, 许 琳, 李文利, 马师洋, 钮自宇, 王 青张爱军, 许琳, 李文利, 马师洋, 钮自宇, 青岛市市立医院消化科 山东省青岛市 266011王青, 青岛市市立医院东区消化科 山东省青岛市 266071作者贡献分布: 此课题由张爱军, 许琳及王青设计; 研究过程由张爱军, 许琳, 李文利, 马师洋及钮自宇操作完成; 研究所用新试剂及分析工具由张爱军与王青提供; 数据分析由张爱军, 许琳及李文利完成; 本论文写作由张爱军完成.通讯作者: 王青, 266071, 山东省青岛市东海中路5号, 青岛市市立医院东区消化科. qingwangqingdao@电话: 0532稿日期: 2008-12-25 修回日期: 2009-01-25接受日期: 2009-02-09 在线出版日期: 2009-03-08 Signifi cance of DNA ploidyanalysis and carcinoembryonic antigen assay for differential diagnosis between benign and malignant ascites Ai-Jun Zhang, Lin Xu, Wen-Li Li, Shi-Yang Ma,Zi-Yu Niu, Qing WangAi-Jun Zhang, Lin Xu, Wen-Li Li, Shi-Yang Ma, Zi-Yu Niu, Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao 266011, Shandong Province, China Qing Wang, Department of Gastroenterology, Qingdao Municipal Hospital East Branch, Qingdao 266071, Shandong Province, China Correspondence to: Qing Wang, Department of Gastroenterology, Qingdao Municipal Hospital East Branch, 5 Donghai Middle Road, Qingdao 266071, Shandong Province, China. qingwangqingdao@ Received: 2008-12-25 Revised: 2009-01-25 Accepted: 2009-02-09 Published online: 2009-03-08 Abstract AIM: To investigate the differential diagnostic signifi cance of DNA ploidy analysis and serum, ascetic carcinoembryonic antigen (CEA), ascites/serum CEA ratios assay in malignant (PHC and non-PHC malignancy) and benign ascites. METHODS: Flow cytometry (FCM) was used for DNA ploidy analysis and enzyme linked immunosorbent assay (ELISA) for CEA assay in 56 malignant (31 PHC and 25 non-PHC) ascites. As a control, 50 nonmalignant ascites were evaluated at the same time. If the serum or ascitic CEA value 5 μg/L, and the ascites/serum CEA ratios 1, then the CEA assay was defined as positive. If an aneuploidy was detected, the result of the DNA ploidy analysis was defi ned as positive. RESULTS: The sensitivity and specificity of ascitic CEA, ascit