GeneXpert技术及原理.pptxVIP

GeneXpert 技术及原理 真正的开始? Cepheid – Proprietary and Confidential重新定义分子诊断The GeneXpertFamily of SystemsGeneXpert ModuleGeneXpert? CartridgeSmartCyclerSmartCycler? Tube SystemICORE?ModuleAny TestAny TimeAny SampleAny Place? Cepheid – Proprietary and Confidential分子诊断检测（PCR）包含以下步骤：扩增样本采集样本处理报告结果检测仪器平台, 耗材 软件SmartCycler SystemGeneXpert System1? Cepheid – Proprietary ConfidentialSmart Cycler? Cepheid – Proprietary and ConfidentialSmartCycler II? Cepheid – Proprietary and ConfidentialSmartCycler? 反应管25 ?l不会损坏！? Cepheid – Proprietary and Confidential一次性反应管最优化的设计:快速温度响应最佳的荧光信号预防污染Sealable pressure capLarge Surface Temperature Area with thin wall enclosureOpticReflectorsPolished Opticwindows(with 90° optics path)? Cepheid – Proprietary and ConfidentialDisposable Tube compensates ClumsinessPAGE9 | ? Cepheid – Proprietary and Confidential9固定的组件BioRadRocheQIAGENPAGE12| ? Cepheid – Proprietary and Confidential12系统模块任意配置Multiple I-COREModules As System Building Blocks? Cepheid – Proprietary and Confidential灵活性: 荧光及模块Smart EBVASR HSVFCTCFATASmart CMV FCTCASR EVFATA? Cepheid – Proprietary and ConfidentialSmartMixTM HMMaster Mix for a PCR assayBufferMgCl2Taq PolymerasedNTP mixturePCR反应PrimersProbeIC扩增/检测? Cepheid – Proprietary and Confidential预混珠? Cepheid – Proprietary and Confidential预混珠形式避免加样误差Reconstitute with WaterAdd the sampleStart the Run? Cepheid – Proprietary and Confidential诊断软件 : DX 3.03 Assay TypesResearchReference IVD? Cepheid – Proprietary and ConfidentialGeneXpert系统? Cepheid – Proprietary and ConfidentialGeneXpert 技术整合的微流体技术，包括：仪器平台试剂盒运行程序和对照? Cepheid – Proprietary and ConfidentialGeneXpert 设计实时荧光定量PCR (扩增和检测同时进行)仪器和样品盒之间不存在湿式接触界面，以消除污染反应体系完全内部质控-不需要设置独立的外部阳性和阴性对照集成的细胞超声裂解释放DNA的装置GeneXpert软件指示单个模块主板以协调阀驱动和整体液压传动 精巧的流体学技术 液体流动由微型阀控制 适于试剂成分的微量反应全自动的数据分析和结果解读? Cepheid – Proprietary and Confidential赛沛的技术GeneXpert 洗涤DNA or RNA提取DNA or RNA纯化 患者样本PCR反应体系SmartCycler 阳性分析检测扩增 阴性? Cepheid – Proprietary and Confidential赛沛的技术GeneXpertSmartCycler? Cepheid – Proprietar