pYES2-DEST52载体说明.docxVIP

pYES-DEST52 编号 载体名称 北京华越洋生物VECT2040 pYES-DEST52 pYES2-DEST52载体基本信息 出品公司: Invitrigen 载体名称: pYES-DEST52 质粒类型: 酿酒酵母表达载体；Gateway载体 高拷贝/低拷贝: 高拷贝 启动子: GAL1 克隆方法: Gateway 载体大小: 7621bp 5 测序引物及序列: -- 3 测序引物及序列: -- 载体标签: His Tag (6x) ; V5 Epitope Tag 载体抗性: 氨苄青霉素、氯霉素（仅空载体） 筛选标记: 报告基因URA3 克隆菌株: DB3.1(空载体的复制） 备注: -- 产品目录号: 12286-019 稳定性: 稳表达 组成型/诱导型: 组成型 病毒/非病毒: 非病毒 载体质粒图谱和多克隆位点信息 pYES2-DEST52载体简介 The pYES2-DEST52 Gateway destination vector is designed for rapid cloning with a Gateway entry clone using lambda phage site-specific recombination and subsequent expression in Saccharomyces cerevisiae. As part of the YES Vector Collection, pYES2-DEST52 carries the promoter and enhancer sequences from the GAL1 gene for regulated expression in S.cerevisiae. pYES2-DEST52 features: 2μ origin of replication for high-copy maintenance gene for auxotrophic selection on economical minimal medium C-terminal V5 epitope and polyhistidine(6xHis) tags for efficient detection and purification tR sites for efficient recombination with any attL-flanked Gateway entry vector 载体序列 ACGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTC ACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATA CTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAAT CAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGA AGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTT CAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAAAATTGTTAATATAC CTCTATACTTTAACGTCAAGGAGAAAAAACCCCGGATCGGACTACTAGCAGCTGTAATACGACTCACTAT AGGGAATATTAAGCTATCAAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATAT CAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCA CTATGGCGGCCGCGGGTGATGCTGCCAACTTAGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGC TTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAA ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGG CATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGAC CGTAAAGAAAAATAAG