补肾通络颗粒对膝骨关节炎大鼠血清细胞因子及关节软骨含水率的影响.docVIP

补肾通络颗粒对膝骨关节炎大鼠血清细胞因子及关节软骨含水率的影响 　　【摘 要】目的：观察补肾通络颗粒高、中、低剂量对膝骨关节炎大鼠血清白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α（TNF-α）水平以及关节软骨含水率的影响。方法：将60只大鼠随机分为空白对照组，模型对照组，补肾通络颗粒高、中、低剂量组，美洛昔康组，每组10只。除空白对照组外，其余各组均予膝关节腔注射木瓜蛋白酶造模，并予药物干预后，用ELISA法测定大鼠血清IL-1β、IL-6、TNF-α的水平，同时光镜下观察关节软骨并检测软骨含水率。结果：实验结束后，根据病理组织学检测发现模型对照组大鼠膝关节软骨退行性改变明显。与空白对照组比较，模型对照组膝骨关节炎大鼠血清IL-1β、IL-6、TNF-α水平及关节软骨含水率均升高（P 0.05）。结论：补肾通络颗粒可能通过调节大鼠膝骨关节炎动物模型血清细胞因子水平及降低关节软骨含水率达到治疗作用，尤以中、高剂量组作用显著。 　　【关键词】 骨关节炎，膝；补肾通络颗粒；大鼠；细胞因子；软骨含水率 　　doi：10.3969/j.issn.2095-4174.2015.10.001 　　【ABSTRACT】Objective：To investigate the effect of Bushen Tongluo Granule （补肾通络颗粒） on the levels of IL-1β，IL-6 and TNF-α and water ratio of articular cartilage in rats with knee osteoarthritis.Methods：60 rats were randomly divided into a blank control group，a model control group，a high-dose Bushen Tongluo Granule group （HBTGG），a medium-dose Bushen Tongluo Granule group （MBTGG），a low-dose Bushen Tongluo Granule group （LBTGG），and a meloxicam group，10 rats in each group.Except for the blank control group，the other groups were modeled by injecting papain into joint cavity.After medicinal intervention，ELISA was used to measure the levels of IL-1β，IL-6 and TNF-α.At the same time，articular cartilage was observed and its water ratio was measured.Results：After experiment，the knee joint cartilage degeneration was obvious in the model group. 　　Compared with the blank control group，the levels of IL-1β，IL-6 and TNF-α and the water ratio of articular cartilage in the model group increased （P 0.05）.Conclusion：Bushen Tongluo Granule may treat knee osteoarthritis by regulating levels of serum cytokines and reducing water ratio of the articular cartilage，especially in the middle and high dose groups. 　　【Keywords】ostoarthritis，knee；Bushen Tongluo Granule（补肾通络颗粒）；rat；cytokine；water ratio of the articular cartilage 　　膝骨关节炎（knee osteoarthritis，KOA）是一种以膝关节软骨退变和关节周围形成骨质增生为病理特征的关节炎，是最常见的一种慢性、进行性、致残性关节疾病。根据流行病学调查，我国KOA患病率在60岁以上人群可达50%，而75岁以上人群则高达80%[1-2]。目前现代医学对于KOA的治疗仍以口服非甾体类抗炎药，或关节腔注射透