水涝胁迫下欧李丙二醛和氧自由基的变化.docVIP

水涝胁迫下欧李丙二醛和氧自由基的变化 　　摘 要：对水涝胁迫下欧李丙二醛和氧自由基含量变化进行初步研究，以期获得欧李植物在成都地区的生长情况，为探讨欧李植物的抗逆性提供理论基础。以双组份分光度法测定丙二醛的含量，提取液与TBA在沸水浴中反应后，分别测定450，532和600 nm波长下的吸光度值;利用O-2.与羟胺反应生成NO2-，NO2-在对氨基苯磺酸和a-萘胺作用下，生成粉红色的偶氮染料，检测植物叶片中O-2.含量。结果显示，水涝胁迫下欧李生长期中，丙二醛含量呈上升趋势;水涝胁迫下欧李氧自由基含量也增加。研究认为随水涝胁迫程度的增加，欧李内氧自由基含量增加，氧化脂膜，导致丙二醛含量增加。 　　关键词：欧李;丙二醛;膜脂过氧化;氧自由基;水涝 　　中图分类号：S793.9 文献标识码：A DOI 编码：10.3969/j.issn.1006-6500.2015.12.004 　　Changes of Malondialdehyde and Oxygen Free Radicals in Prunus humilis Bunge Leaves under Waterlogging Stess 　　ZHANG Hai-ping1， CHENG Xiao-ai2 　　（1. Dingxiang County State Forest， Shanxi Province， Dingxiang， Shanxi 035400， China; 2. Taigu County Agricultural Commission， Shanxi Province， Taigu， Shanxi 030800， China） 　　Abstract： MDA and oxygen free radicals were determined to obtain the growing state of Prunus humilis Bunge under waterlogging stress and provide theoretical basis for stress tolerance of Prunus humilis Bunge in Chengdu. The content of MDA was detected by two-component spectrophotometry method. The absorbance at 450， 532 and 600 nm were determined after the extract and TBA reacted in the boiling water bath; the content of O2-. was detected through pink azo dyestuffs which were obtained by the reaction of O2-. and hydroxylamine to gain NO2-， NO2- then reacted with sulfanilic acid and a-naphthalene amine. The results showed that the content of MDA and O2-. were all increased during the waterloggin. The contents of oxygen free radicals increased and caused bilayer lipid membrane oxidation; the contents of MDA also increased during the waterlogging because MDA was the product of bilayer lipid membrane oxidation. 　　Key words： Prunus humilis Bunge; malondialdehyde; membrane lipid peroxidation; oxygen free radicals; waterlogging 　　欧李[1]（又称钙果）Prunus humilis Bunge为蔷薇科樱桃属矮生樱桃亚属的一种野生灌木果树，主要分布在中西部的山西、河北、陕西、内蒙等干旱地区。植株高0.3～1.5 m，抗寒，耐旱，适应性强。经济价值极高，用途非常广泛。可食用，仁可入药，茎可作饲料。果实呈红色，呈扁圆形、枣圆形、尖桩形不等，酸甜适中，味道可口，富含维生素A、维生素B2、维生素B12、维生素C、维生素E，微量元素氮、钾、钙、铁、锰、镁、锌、硒等，而且含有多种氨基酸，果实含糖量可