study report The water permeability of Arabidopsis plasma membrane is regulated.pptVIP

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study report The water permeability of Arabidopsis plasma membrane is regulated.ppt

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study report The water permeability of Arabidopsis plasma membrane is regulated

The water permeability of Arabidopsis plasma membrane is regulated by divalent cations and pH Patricia Gerbeau, Gabriela Amodeo, Tobias Henzler, Veronique Santoni, Pierre Ripoche and Christophe Maurel Author Christophe Maurel Principal investigator of the Aquaporin team in Biochimie et Physiologie Moleculaire des Plantes, France Veronique Santoni Permanent staff of the Aquaporin team There was a cruise ship full of English, French, Germans and Italians, sinking in the middle of the Med. The captain had radioed for help and been told that if the passengers jumped into the sea then they could be picked up. The captain sent his young second-in-command to give the passengers their instructions. 5 minutes later he came back looking disappointed. Whats wrong? the captain asked. They all refuse to jump into the sea. They wont listen to me, replied his second-in-command. So, the captain went off to do it himself. He came back 5 minutes later. However, he returned a smug grin! How did you do it? asked the second. Well... started the captain, I told the British it was traditional. I told the French it was fashionable. I told the Germans it was an order, and I told the Italians it was forbidden! The Aquaporin team Aquaporin team aims at understanding the integrated function of the numerous aquaporin isoforms present in plants, using Arabidopsis thaliana as a model species. In recent years, the Aquaporin team has investigated the variety of molecular and cellular mechanisms involved in aquaporin regulation Tournaire-Roux C, M Sutka, H Javot, E Gout, P Gerbeau, D-T Luu, R Bligny, C Maurel (2003) Cytosolic pH regulates root water transport during anoxic stress through gating of aquaporins. Nature, 425: 393-397. Aqueous Two-phase System, ATPS Summary Cell pressure probe Cell hydraulic conductivity Aqueous two-phase partitioning Isolate PM vesicles Stopped-flow spectrophotometry Characterize their water transport properties Experiment Summary Alteration