东南大学电子学院《现代光学基础》补充内容3——显微镜资料.pptVIP

第七章 共焦显微光学Chapter 7 Confocal Microscopy Optics;OUTLINE;7.1 Optical Microscopy;Microscopy: History;Microscopy: History;Microscopy: History;Microscopy: Importance;Simple Magnification;Image formation on Retina;Combination of Lens and Eye;Combination of Lens and Eye;Compound Microscope;Compound Microscope;Airy disks and resolution. (a-c) Airy disk size and related intensity profile (point spread function) as related to objective numerical aperture, which decreases from (a) to (c) as numerical aperture increases. (e) Two Airy disks so close together that their central spots overlap. (d) Airy disks at the limit of resolution.;;7.2 Confocal Microscopy;Principle of Confocal Microscopy ;;;;Principle of CM;;;;;Lasers;;Pinhole ;Scanning System;Detectors;Resolution and Contrast;Resolution and Contrast;Resolution and Contrast;Resolution and Contrast;Resolution and Contrast;Resolution and Contrast;Resolution and Contrast;Benefits of Confocal Microscopy;Drawbacks of Confocal Microscopy;Historical Perspective ;Historical Perspective;Modern confocal microscopes ;Modern confocal microscopes;;7.3 Applications in Biomedicine;荧光探针（Fluorescent probe） ;Fluorescence Excitation and Emission Fundamentals ;Timescale Range for Fluorescence Processes;Stokes Shift and Mirror Image Rule;What is TPA?;Applications of TPA;双光子荧光成像 （续）;量子点 又称半导体纳米微晶粒，直径在1－100nm之间，能够吸收激发光产生荧光的半导体纳米颗粒 ??????;量子点的结构（续）;量子点的性质;我们制备的不同尺寸的CdSe量子点在同一激发波长（365 nm）下发出的荧光。 ;样品（上图左起2、4、6）的紫外吸收光谱以及相应的荧光发射光谱（PL） ;Applications;细胞的三维重建（3-D Reconstruction）;;;;细胞定量荧光测定;细胞内钙离子PH值和其它离子的动态分析;细胞胞间通讯（Cell Communication）和膜的流动性;荧光光漂白恢复（Fluorescence Redistribution After Photo bleaching，FRAP）;;笼锁-解笼锁测定（Caged-Uncaged）;粘附细胞分选（Adhered Cell Sort）和显微手术（Micro-operation）;激光光陷阱技术（Optical trap）;表面增强Raman谱（SERS）;;基于SERS的纳米pH传感器;基于SERS的纳米pH传感器;基于SERS的纳米pH传感器;基于SERS的纳米pH传感器;