蛇床子素对肿瘤细胞AGS中糖基转移酶β3Gn-T2其相关基因表达影响.docVIP

蛇床子素对肿瘤细胞AGS中糖基转移酶β3Gn-T2及其相关基因表达的影响 摘 要 目的 胃癌是我国常见的恶性肿瘤之一，其发病率居各类肿瘤的首位β1，3糖基转移酶(β3GnT8即β3GalT7)是本实验室通过电子克隆得到的一个新型的人类糖基转移酶，通过同源序列比对确定其属于β3糖基转移酶大家族。β3GnT2mRNA水平表达的变化，在24h 48h 后RT-PCR检测 β3GnT2 mRNA的表达。 结果 随着药物浓度和时间增加细胞株AGS β3GnT2的表达轻度降低. 但在高药物浓度时，细胞株AGS β3GnT2在48h比24h的表达有所提高。 结论 （1） 蛇床子素可能通过下调肿瘤细胞中GnT2的表达影响细胞表面糖链结构，这种改变可能与蛇床子素诱导的肿瘤细胞凋亡有关。（2）肿瘤细胞对蛇床子素可能具有一定的耐药性 关键词：蛇床子素 ；β3Gn-T2；AGS Study on the effect of Osthole to the β3Gn-T2 and its related genes in Tumor Cell Lines AGS Abstract: Objectives：Gastric cancer is one of common malignant tumors in China, the incidence in our country ranks first in all tumor Osthole can kill tumor cells.β1,3-N-acetylglucosaminyltransferase 8 is a new member of glycosyltransferases we gained by electric cloning. We considered it belongs to beta-3 glycosyltransferase family.This research is focus on the relation between Osthole and β3Gn-T2. Methods：To study the variation of β3Gn-T2 and its related genes expression in AGS cell lines after Osthole added to the culture medium． Use RT-PCR to detect the mRNA expression of GnT2 in AGS cell line Results：After Osthole added to the culture medium，the mRNA expressions of GnT2, in AGS cell line decreased, but at the high drug concentration，the cell line AGS β3GnT2 in 48h have more expression than in 24h Conclusion：（1）This study showed that the expression of GnT2 are different in the cell line after Osthole added to the culture medium．This result suggested that the effect of Osthole maybe change the sugar chains of glycoprotein in cell surface. （2）tumor cells may have some resistance towards Osthole Key Words 　 Osthole ; β3GnT2；AGS Written by : Lijun Shen Supervised by : Shiliang Wu ………………………………………………………………… 1 2 蛇床子素对肿瘤细胞中β3Gn-T2及其相关基因mRNA表达的影响…………………………………… 6 2.1 实验方法………………………………………………………… 6 2.2 实验结果………………………………………………………… 9 3讨论………………………………………………………………… 11 4 缩略词表…………………………………………………………… 13 5 致谢………………………………………………………………… 15 前言 　 蛇床子系伞形科蛇床子属植物蛇床的果