GenBank数据库中黄麻EST-SSR标记的开发及其通用性评价.pdfVIP

作物学报ACTAAGRONOMICASINICA 2014，40(7)：1213—1219 http：／／zwxb．chinacrops．org／ ISSN TSHPA9 0496-3490；CODEN DoI：10．3724／SP．J．1006．2014．01213 张立武” 袁民航” 何雄威 刘 星 方平平 林荔辉 陶爱芬 徐建堂 祁建民+ 福建农林大学作物科学学院／作物遗传育种与综合利用教育部重点实验室，福建福州350002 摘 用Primer 开发效率较高，不仅可以丰富黄麻分子标记的数量，而且为剖析黄麻重要性状的遗传机制奠定基础，这对于黄麻的 遗传基础研究具有重要应用价值。 关键词：黄麻；EST；SSR；标记开发：多态性 and Evaluationof Markersfrom DevelopmentUniversality EST-SSR in Jute ZHANG Li．Wu’+，YUAN Li—Hui，TAO Min—Hang’+，HEXiong—Wei，LIUXing，FANGPing—Ping，LIN Jian—Min‘ Ai—Fen，XUJian—Tang，andQI for and Utilizationof of of of and Laboratory Ministry Agriculture Key Genetics，BreedingMultiple Crops Education／CollegeCropScience，Fujian ForestryUniversity,Fuzhou350002，China Abstract：Inthis totalof838 werederivedfrom softwarePl佃er study,a iuteexpressedsequencetags(ESn GenBank．Using 3．0， 66 of were basedonthe screenedtheonline SSRPrimer． pairsprimersdesigned sequencerepeat(ssR、sitesby simple program These were insix accessionsofdifferenttotesttheir primersamplifiedjute types polymorphism．111eagarosegelelectrophoresis resultsshowedthat42 of betweenatleasttwoaccessionsandthePCR were pairsprimers(63．6％、werepolymorphic products clearandstable EST-SSRscontainedrich couldbeconsideredasthe fragment．These motif(AT)nor(AC-)n，which polymorphic dominantmotifin iseffectiveto SSRmarkersin fromESTs inGenBank．The