SPR技术..ppt

SPR-MS Anal. Chem. 2000, 72,4193-4198 SPR-MS Anal. Chem. (2000) 404 A-411 A 微型化 Biacore 2000 Dimensions: 760 x 350 x 610 mm Net Weight: 50 kg Spreeta 2000 微流控SPR芯片 The Integrated microfluidics Cartridges used in the Biacore?3000 SPR的应用 对生物分子进行识别及定量检测 研究生物分子间的相互作用 用SPR可获得的信息： 两个分子之间结合的特异性 目标分子的浓度 结合以及解离过程的动力学参数 结合的强度 Elucidate the function of novel proteins in proteomics research Identify binding partners to any target molecule – ligand fishing Determine the nature of protein complexes and their function Unravel the intricate molecular interactions involved in cell signaling pathways Study membrane associated molecules in near native environments Applications in life science research Applications in life science research Determine expression levels of selected proteins under different conditions, for example, health, disease, drug treatment Investigate the binding properties of protein isoforms Find antagonists to critical binding events Identify disease markers in clinical samples Optimize therapeutic antibodies Evaluate ion selectivity in signaling pathways Identify DNA damage – mutations, DNA adducts etc Applications in Drug Discovery Proteomics – target identification and ligand fishing Target and assay validation for High Throughput Screening (HTS) ‘Hit’ to lead characterization – rapid affinity ranking and detailed kinetics of interaction for small molecules binding to target proteins Lead optimization through interaction kinetic based QSAR (Quantitative Structure Activity Relationship) Early ADME (Absorption, Distribution, Metabolism, Excretion) – rapid in vitro assays for defined ADME parameters, such as protein binding studies Clinical trials – stratification of patient response to therapeutics Production and manufacturing – process and batch QC of therapeutic antibodies and proteins Applications in Food Analysis Quality control — Rapid and reliable vitamin analysis in health and nutrition food manufacturing Food Safety — Reliable screening for veterinary