《RNA干扰水稻SBE3基因的表达对籽粒淀粉合成及其关键酶活性的影响》.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2010, 36 2 : 313?320 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.00313 RNA 干扰水稻 SBE3 基因的表达对籽粒淀粉合成及其关键酶活性的 影响 汪结明 张 建 江海洋 朱苏文 范 军 程备久* , 230036 : RNA SBE3 , 11 , SBE3 , SBE3 RNA , 3 d , ADPG-PPase SSS DBE , SSS ADPG-PPase , , , , : ; RNA ; 3; ; Effects of RNA Interference of SBE3 Gene Expression on Starch Accumulation and Key Enzymes Activities Involved in Starch Synthesis in Transgenic Rice Grain * WANG Jie-Ming, ZHANG Jian, JIANG Hai-Yang, ZHU Su-Wen, FAN Jun, and CHENG Bei-Jiu Anhui Agricultural University, Anhui Provincial Key Laboratory of Crop Biology, Hefei 230036, China Abstract: Rice cultivar “Zhonghua 11” control and its transgenic line were used as the experimental materials. The expression level of SBE3 gene, some related key enzymes activities, and the contents of amylose and amylopectin at different developmental stages of rice grain were measured. Results showed that the SBE3 gene expression level and the SBE activity were distinctly re- duced by RNA interference RNAi , but a little difference was observed between the two transgenic lines. The peak values of SBE activity reached three days earlier in transgenic rice than in non-transgenic control. RNAi of the SBE3 gene expression level also obviously reduced the enzyme activities of ADPG-PPase, SSS and DBE during different grain developmental stages, especially, the peak values of activities of ADPG-PPase and SSS decreased to the largest extent. In addition, the content of amylose was sig- nificantly higher at different developmental stages in transgenic rice grain than that in the control, while thousand-grain weight was significantly reduced in mature grain, the general trend was that the higher the amylose content, the smaller the grain weight. Keywords: Rice; RNA interference; Starch branching enzyme 3; Starch accumulation; Enzyme activity Oryza sativa L. 1 Q- starch branching en- ,