真菌Marasmiustricolor310b的培养特性及发酵产漆酶条件的优化论文.docVIP

真菌Marasmius tricolor310b的培养特性及发酵产漆酶条件的优化 姬蓉蓉 （青海大学农牧学院草业科学系，青海西宁 810016） 摘要 以一株来自高寒草地土壤纤维素分解真菌Marasmius tricolor310b为研究对象，对其在不同温度、不同碳源、不同氮源、不同盐浓度、不同pH、不同盐离子等不同条件下培养，通过十字交叉法测定菌株直径，从而确定该菌株活性最大时的生物学特性;应用摇瓶液体发酵的方法，以秸秆粉为底物分别在不同培养条件下（温度、碳源、氮源、盐浓度、pH、盐离子），按照王剑锋等的方法进行测定其酶活的大小。经初步研究，结果表明菌株Marasmius tricolor310b在30℃、初始pH为4.8（偏酸性）、蔗糖为碳源、蛋白胨为氮源、盐浓度≤3％时生长速度最快。 关键词：漆酶 ；真菌； 生物学特性 Rongrong-ji (Department of Grassland Science, Agriculture and Animal Husbandry College, Qinghai University, Qinghai Xining, 810016) Abstract In alpine meadow soil cellulose decomposition fungi strains from marasmius tricolor310b as the research object,in the different temperature,different carbon source ,nitrogen source,different salt concentration,pH，salt ions under different conditions,such as cultivating,strain diameter can be determined through cross the crossover,to determine the biological characteristics of the strain when the most reactive;shake bottle of liquid fermentation method,straw powder as substrate respectively in different culture conditions(temperature、concentration of carbon source、nitrogen source、nitrogen source、salt、pH、salt ions),according to the Jian-feng wang and other methods for determination of the size of the enzyme activity.A preliminary study,result show that the strain marasmius tricolor310b at 30 degrees,the initial pH 4.8 (partial acid),sucrose as carbon source and peptone and nitrogen source,grow fastest when salt concentration of 3％ or less. Keywords:laccase;fungi;biological characteristics 目录 1绪 论 1 1.1 研究目的与意义 1 1.2 国内外研究概况 1 5℃ 10℃ 15℃ 20℃ 25℃ 30℃ 35℃ 2014.11.9 0.6 0.6 2014.11.10 0.6 0.6 2.14.11.11 0.6 0.6 2014.11.12 0.6 0.6 2014.11.13 0.6 0.6 2014.11.14 0.6 0.6 2014.11.15 0