Overexpression of a minor component of myelin basic protein isoform (17.2 kDa) can restore myelinogenesis in transgenic shiverer mice》.pdf

Brain Research 785 1998 245–252 Research report Overexpression of a minor component of myelin basic protein isoform 17.2 kDa can restore myelinogenesis in transgenic shierer mice Minoru Kimura a, , Masahiro Sato a,1 , Akira Akatsuka b , Shigeharu Saito a,2 , Kiyoshi Ando a,c , Minesuke Yokoyama d,3 , Motoya Katsuki a,4 a Diision of Molecular Life Science, School of Medicine, Tokai Uniersity, Bohseidai, Isehara, Kanagawa 259-11, Japan b Electron Microscopy Section of Interdepartmental Laboratories, School of Medicine, Tokai Uniersity, Bohseidai, Isehara, Kanagawa 259-11, Japan c Diision of Microbiology, School of Medicine, Tokai Uniersity, Bohseidai, Isehara, Kanagawa 259-11, Japan d Central Institute for Experimental Animals, 1430 Nogawa, Miyamae, Kawasaki, Japan Accepted 11 November 1997 Abstract Shierer shi mice, which are neurologically mutant, lack a large portion of the gene for the myelin basic proteins MBPs , have virtually no myelin in their central nervous system CNS , and shiver, undergo seizures, and die early. At least five types of MBPs 21.5, 18.5, 17.3, 17.2 and 14.0 kDa are known to be generated through alternative splicing from a single MBP gene. We have produced transgenic shi mice carrying a cDNA encoding mouse 14-kDa MBP isoform, the most abundant form of MBPs, under control of a