Myelin deficient (shimld) mutant allele Morphological comparison with shiverer (shi) allele on a B6C3 mouse stock》.pdf

Brain Research, 360 (1985)235-247 235 Elsevier BRE 11269 Myelin Deficient (shimid) Mutant Allele: Morphological Comparison with Shiverer (shi) Allele on a B6C3 Mouse Stock XIN-YA SHEN1.3, SUSAN BILLINGS-GAGLIARDI1,RICHARD L. SIDMAN2and MERRILL K. WOLF 1 1Department of Anatomy, University of Massachusetts Medical School, Worcester, MA 01605, 2Department of Neuroscience, Childrens Hospital, Boston, MA 02115 (U.S.A.) and 3Department ofAnatomy, Shanghai First Medical College, Shanghai (Peoples Republic of China) (Accepted April 2nd, 1985) Key words: myelin - - genetic mutation - - oligodendrocyte - - tissue culture A new B6C3 stock of shiratamutant mice is compared in terms of behavior and CNS morphology with both a B6C3 shi stock and re- ports on other shirataanimals. Defects of B6C3 shiratamyelination seen at postnatal day 21 (P-21) are comparable to those in B6C3 shi with respect to % axons myelinated, sheath thickness, errors in the wrapping and targeting of myelin and abnormal oligodendrocyte shape. The two mutations are similarly expressed in cerebellar organotypic cultures. However, the major dense line (MDL) is present in a few shi aamyelin sheaths at P-21 and a few sheaths show myelin basic protein by immunocytochemistry, while neither phenome- non is seen in shi at this age in the same CNS regions. Shimidmice survive their disease significantlybetter than shi. The shiratastock cur- rently under study elsewhere differs from this B6C3 stock in that MDL was reported only in older animals, and behavior and survival were severely