Nicotinic acid induces apolipoprotein A-I gene expression in HepG2 and Caco-2 cell lines》.pdfVIP

M E T A B O L I S M C L I N I C A L A N D E X P E R I M E N T A L 6 0 ( 2 0 1 1 ) 1 7 9 0 – 1 7 9 6 A v a i l a b l e a t w w w . s c i e n c e d i r e c t . c o m Metabolism w w w . m e t a b o l i s m j o u r n a l . c o m Nicotinic acid induces apolipoprotein A-I gene expression in HepG2 and Caco-2 cell lines Michael J. Haasa, Abdul-Razzak Alamira, Senan Sultana, Joe M. Chehadea, Norman C.N. Wongb, Arshag D. Mooradiana,⁎ a Division of Endocrinology, Department of Medicine, University of Florida College of Medicine, Jacksonville, FL 32209, USA b Division of Endocrinology, Department of Medicine University of Calgary, Calgary, Alberta, T2N 4N1 Canada A R T I C L E I N F O A B S T R A C T Article history: The objective was to test the effect of nicotinic acid on apolipoprotein A-I (apo A-I) gene Received 1 February 2011 expression in hepatic (HepG2) and intestinal (Caco-2) cell lines. HepG2 and Caco-2 cells Accepted 3 May 2011 were treated with 0.1, 0.3, 1.0, 3.0, and 10 mmol/L of nicotinic acid; and apo A-I concentrations in conditioned media were measured with Western blots. Relative apo A-I messenger RNA (mRNA) levels, normalized to glyceraldehyde-3-phosphate dehydrogenase mRNA, were measured with quantitative real-time polymerase chain reaction method. The nicotinic acid response element in the apo A-I