Lecithin cholesterol acyltransferase (LCAT) activity in the presence of Apo-AI-derived peptides exposed to disorder–order conformational transitions》.pdfVIP

Biochemical and Biophysical Research Communications 441 (2013) 469–475 Contents lists available at ScienceDirect Biochemical and Biophysical Research Communications journal homepage: www.else /locate/ybbrc Lecithin cholesterol acyltransferase (LCAT) activity in the presence of Apo-AI-derived peptides exposed to disorder–order conformational transitions S.L. Aguilar-Espinosa a, P. Mendoza-Espinosa a, B. Delgado-Coello a, J. Mas-Oliva a,b,⇑ a Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico DF, Mexico b División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 Mexico DF, Mexico a r t i c l e i n f o a b s t r a c t Article history: Although the association of Apo AI with HDLs has been proposed to activate LCAT activity, the detailed Received 9 October 2013 molecular mechanisms involved in the process are not known. Therefore, in this study we have investi- Available online 26 October 2013 gated how conformational changes in several exposed regions of Apo-AI might cause LCAT activation and for this purpose, designed a strategy to investigate three Apo AI-derived peptides. Since these peptides Keywords: present the ability to adopt several secondary structure conformations, they were used to determine Plasma LCAT activity whether LCAT activity could be modulated in the presence of a particular conformation. Circular dichro- Apo-AI