Interaction of thrombospondin1 and CD36 contributes to obesity-associated podocytopathy》.pdfVIP

Biochimica et Biophysica Acta 1852 (2015) 1323–1333 Contents lists available at ScienceDirect Biochimica et Biophysica Acta j ournal homepage: www.el sevier. com/l ocate/ bb adis Interaction of thrombospondin1 and CD36 contributes to obesity-associated podocytopathy Wenpeng Cui a,b, Hasiyeti Maimaitiyiming a,c, Qi Zhou a,c, Heather Norman a,c, Changcheng Zhou a, Shuxia Wang a,c,⁎ a Department of Pharmacology and Nutritional Sciences, University of Kentucky, Lexington, KY 40536, USA b Department of Nephrology, Second Hospital of Jilin University, Changchun 130041, China c Lexington Veterans Affairs Medical Center, Lexington, KY, USA a r t i c l e i n f o a b s t r a c t Article history: Obesity is associated with podocyte injury and the development of proteinuria. Elevated plasma free fatty acid is Received 1 October 2014 one of the characteristics of obesity and has been linked to podocyte dysfunction. However, the mechanisms re- Received in revised form 19 March 2015 main unclear. In the current study, we examined the effect of saturated free fatty acid (FFA) on human podocyte Accepted 23 March 2015 apoptosis and function in vitro. The mechanism and its in vivo relevance were also determined. We found that Available online 31 March 2015 FFA treatment induced human podocyte apoptosis and dysfunction, which was associated with increased expres- sion of a matricellular protein-thrombospondin1 (TSP1). FFA stimulat