Identification and characterization of MAVS from black carp Mylopharyngodon piceus》.pdfVIP

Fish Shellfish Immunology 43 (2015) 460e468 Contents lists available at ScienceDirect Fish Shellfish Immunology journal homepage: www.elsevier.c om/locate/fsi Full length article Identification and characterization of MAVS from black carp Mylopharyngodon piceus Wei Zhou a, Jujun Zhou a, Ying Lv a, Yixiao Qu a, Mengdie Chi a, Jun Li a, Hao Feng a, b, * a Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education of China, College of Life Science, Hunan Normal University, Changsha, 410081, China b State Key Laboratory of Freshwater Ecology and Biotechnology, Wuhan, 430072, China a r t i c l e i n f o a b s t r a c t Article history: MAVS (mitochondria antiviral signaling protein) plays an important role in the host cellular innate Received 12 December 2014 immune response against microbial pathogens. In this study, MAVS has been cloned and characterized Received in revised form from black carp (Mylopharyngodon piceus). The full-length cDNA of black carp MAVS (bcMAVS) consists of 14 January 2015 2352 nucleotides and the predicted bcMAVS protein contains 579 amino acids. Structural analysis Accepted 22 January 2015 showed that bcMAVS is composed of functional domains including an N-terminal CARD, a central Available online 3 February 2015 proline-rich domain, a putative TRAF2-binding motif and a C-terminal TM domain, which is similar to