High-level expression of angiotensin converting enzyme inhibitory peptide Tuna AI as tandem multimer in Escherichia coli BL21 (DE3)》.pdfVIP

Process Biochemistry 50 (2015) 545–552 Contents lists available at ScienceDirect Process Biochemistry j o u r n a l h o m e p a g e: w w w. elsev /locate/proc bio High-level expression of angiotensin converting enzyme inhibitory peptide Tuna AI as tandem multimer in Escherichia coli BL21 (DE3) Yunliang Li a , Bei Wang a,c , Henan Zhang a , Zhigao Wang a , Shanying Zhu b , Haile Ma a,∗ a School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, PR China b School of the Environment, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, PR China c Department of Biological and Agricultural Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA a r t i c l e i n f o a b s t r a c t Article history: Food-derived angiotensin converting enzyme inhibitory peptides (ACEIP) show a great promise for pre- Received 15 November 2014 vention and treatment of hypertension. Tuna muscle derived peptide, Tuna AI (PTHIKWGD), is a potent Received in revised form ACEIP with high activity and stability. To improve the expression level of Tuna AI in Escherichia coli, 26 December 2014 multimer genes of Tuna AI were assembled and expressed as fusion proteins by constructing the vec- Accepted 19 January 2015 Available online 2 February 2015 tors of pET30-4nTunaAI (n = 1, 2, 4 and 8). The results showed the multimer genes were successfully expressed in the constructed system, and their expression level im