Glucocorticoids Dose-related effects on osteoclast formation and function via reactive oxygen species and autophagy》.pdfVIP

Bone 79 (2015) 222–232 Contents lists available at ScienceDirect Bone journal homepage: /locate/bone Original Full Length Article Glucocorticoids: Dose-related effects on osteoclast formation and function via reactive oxygen species and autophagy Jun Shi a,1, Long Wang a,1, Hongyang Zhang a,1, Qiang Jie a,1, Xiaojie Li a, Qiyue Shi b, Qiang Huang a, Bo Gao a, Yuehu Han a, Kai Guo a, Jian Liu a,⁎, Liu Yang a,⁎⁎, Zhuojing Luo a,⁎ a Institute of Orthopedics, Xijing Hospital, Fourth Military Medical University, China b Institute of Orthopedics, Xian Chinese Medicine Hospital, China a r t i c l e i n f o a b s t r a c t Article history: Whether glucocorticoids directly enhance or interrupt osteoclastogenesis is still a controversial subject. In this study, Received 10 February 2015 we ascertained the dose-dependent positive effects of glucocorticoids on osteoclastogenesis in vivo and in vitro as Revised 15 June 2015 well as investigated the mechanism in vitro. As the dose of glucocorticoids increased, osteoclastogenesis was stim- Accepted 17 June 2015 ulated at 0.1 μM, a peak was achieved at 1 μM and a corresponding decrease occurred at 10 μM. Reactive oxygen spe- Available online 24 June 2015 cies (ROS), which play a crucial role in osteoclastogenesis, and autophagy flux activity, a cellular recycling process, Edited by: Hong-Hee Kim were consistently up-regulated along