Evaluation of apolipoprotein E secretion by macrophages in type 2 diabetic patients role of HDL and apolipoprotein A-I》.pdfVIP

Diabetes Research and Clinical Practice 69 (2005) 124–128 /locate/diabres Evaluation of apolipoprotein E secretion by macrophages in type 2 diabetic patients: role of HDL and apolipoprotein A-I a,b a,* a a Kazumi Yamato , Naoki Tamasawa , Hiroshi Murakami , Jun Matsui , Jutaro Tanabea a b , Toshihiro Suda , Minoru Yasujima aThird Department of Internal Medicine, Hirosaki University School of Medicine, Zaifu-5, Hirosaki 036-8562, Japan bLaboratory Medicine, Hirosaki University School of Medicine, Zaifu-5, Hirosaki 036-8562, Japan Received 17 August 2004; received in revised form 4 October 2004; accepted 12 November 2004 Available online 28 December 2004 Abstract It has been shown that apolipoprotein A-I (ApoA-I) stimulates the secretion of apolipoprotein E (ApoE) from human macrophages. ApoA-I is a major protein constituent of HDL which because of its role in reverse cholesterol transport, has been implicated in the prevention of atherosclerosis. We herein investigated the ability of monocyte-derived macrophages (MDMs) in 42 patients with type 2 diabetes to secrete ApoE; these patients commonly have low plasma HDL and ApoA-I levels. Our data showed that ApoE secretion from these cells was reduced in patients with low plasma HDL and ApoA-I levels; there were positive correlation between ApoE secretion from MDMs and plasma HDL (r2 = 0.33, p = 0.03) and ApoA-I (r2 = 0.31, p