处理和培养环境对精子DNA完整性的影响.pdfVIP

Asian Journal of Andrology (2010) 12: 753–759 npg © 2010 AJA, SIMM SJTU All rights reserved 1008-682X/10 $ 32.00 753 /aja Original Article Preparation and incubation conditions affect the DNA integrity of ejaculated human spermatozoa Rieko Matsuura, Takumi Takeuchi, Atsumi Yoshida The Reproduction Center, Kiba Park Clinic, Tokyo 135-0042, Japan Abstract Appropriate semen processing and assessment are critical for successful infertility treatment. We investigated whether laboratory procedures including semen preparation and incubation affect sperm DNA integrity. A total of 153 infertile men were involved. Conventional semen parameters and sperm chromatin structure assay (SCSA) parameters, that is, DNA fragmentation index (%DFI) and high DNA stainability (%HDS), were assessed on the fresh ejaculated semen samples, which were treated and incubated under different conditions. Negative correlations were identified between the %DFI and sperm concentration, motility, progressive motility and morphology. A lower percentage of DFI was detected in spermatozoa when density gradient centrifugation (DGC) was followed by swim- up treatment in comparison with DGC alone (P 0.01). Although the %DFI increased in a time-dependent manner with incubation both at room temperature (RT) and at 37ºC in air, the %DFI after 24 h at RT was significantly lower than that at 37ºC (P 0.05). Incubation with 5% CO2 was effective in maintaining sperm motility (P 0.01); however, it induced further elevation of %DFI (P 0.001). Thus, sperm DNA damage was associated with longer incubat