叉枝藻和海带提取物对黄嘌呤氧化酶抑制作用研究.pdf

g/mL 。采用等毒法研究海带水溶性组分与别嘌呤醇的联合抑制作用，结果表明， 水溶性组分与别嘌呤醇的联合抑制作用为协同作用。实验还测定了海带水溶性组 分的自由基清除能力，结果表明，海带水溶性组分对羟自由基和超氧阴离子都有 清除作用。 叉枝藻水溶性组分和乙酸乙酯组分的干物质得率分别为 12.7%和 0.30% ，组 间差异具有统计学意义（P ＜0.01 ）。其IC50 分别为 0.43mg/mL 和 0.51mg/mL 。选 择抑制活性较强，得率较高的水溶性组分进行动力学分析，该组分对黄嘌呤氧化 酶表现为竞争性可逆抑制作用，抑制动力学常数 K =0.37mg/mL 。采用等毒法研 i 究叉枝藻水溶性组分与别嘌呤醇的联合抑制作用，结果表明，该组分与别嘌呤醇 的联合抑制作用为相加作用。实验测定了叉枝藻水溶性组分的自由基清除能力， 结果表明，叉枝藻水溶性组分仅对羟自由基有清除作用。 综合两种海藻对黄嘌呤氧化酶的抑制作用研究，海带和叉枝藻的抑制活性成 分多集中于水溶性组分中。 关键词：海带；叉枝藻；黄嘌呤氧化酶；抑制作用；痛风 II Studies on Inhibition Effects of Extracts from Laminaria japonica Aresch and Gymnogongrus flabelliformis Harv on the Xanthine oxidase Abstract Xanthine oxidase (EC 1.2.3.2， XOD) is a kind of composite flavin enzyme, widely distributed in bacteria, fungi and mammalian tissue. It is a key enzyme in the process of nucleic acid metabolism in vivo , which catalyzes xanthine and hypoxanthine produce uric acid and peroxide radicals. Excessive uric acid concentration would lead to hyperuricemia and gout. In recent years, the XOD inhibitor has been widespread concern of research scholars at home and abroad, as a very effective anti-gout drug. So, the study of screening new naturally XOD inhibitor would provide a theoretical basement for the development of XOD targeting anti-gout drug and health products. This article made a deep research on the XOD inhibition of the ethanol extracts that extracting from Laminaria japonica Aresch and Gymnogongrus flabelliformis Harv . The optimum extracting conditions of the substances from two seaweeds were confirmed by single factor and orthogonal experiment . The inhibitory mechanism and types of the extracts on XOD were studied using