《离子色谱检测氨基酸-苏氨酸》.pdfVIP

离子色谱-电导检测法测定苏氨酸 1，2 ∗2 1 刘春梅 ，丛威 ，张栩 （1.北京化工大学生命科学与技术学院，北京 100029；2.中国科学院过程工程研究所生化工程国家重点 实验室，北京 100190) 摘要 建立了离子色谱-电导检测器直接进样测定苏氨酸的方法。淋洗液为 4.0 mmol/L 的酒石酸和 1.0 mmol/L的2,6-吡啶二羧酸，流速为1.0 mL/min，检测器为电导检测器。结果表明， 苏氨酸在 10 ~ 100 mg/L 的线性范围内线性良好，回归方程为：A = 0.3649 C - 0.3315 ，R2 = 0.9998 ，精密度实验 RSD = 1.393%，最 小检测限为1.8 mg/L；苏氨酸与其它阳离子同时测定时互不干扰；非离子态物质对苏氨酸的测定没有影响。 离子色谱-电导检测器测定方法简单、准确，可用于苏氨酸的定量分析。 关键词 离子色谱，电导检测器，苏氨酸 Determination of Threonine by Ion Chromatography with Conductivity Detector Liu Chun-Mei1,2, Cong Wei*2 ，Zhang Xu1 （１.College of Life Science and Technology，Beijing University of Chemical and Technology,Beijing 100029） ２.State Key Lab of Biochemical Engineering，Institute of Process Engineering ，Chinese Academy of Sciences, Beijing 100190) Abstract A new method was established for determination of threonine( Thr ). Ion chromatography with conductivity detector was adopted with tartaric acid ( 4.0 mmol/L )and 2,6- Pyridinedicarboxylate (1.0 mmol/L ) as the eluent.the flow rate was 1.0 mL/min. The detection was performed by a conductivity detection mode. The regression equation with Thr was A = 0.3649 C - 0.3315 .The calibnation curves were linear with a range of 10 ~ 100 mg/L，R2 = 0.9998 . Precision test RSD was 1.393%.The coinstantaneous determination of Thr and other cations were achieved; non-iron component has no influence with the detemination of threonine. This method is simple,accurate and suitable for the determination of Thr. Key words: Ion Chromatography( IC )；Conductivity Detector ；Threonine( Thr ) 1 引言 苏氨酸[1] 是人与动物体内所必需的一种氨基酸，化学名为：L-α-氨基-β-羟基丁酸 (L-α-Amino-β-hydroxybutyric acid )；分子式：C H NO ，分子量为 119.12