基于SSR标记的荔枝种质遗传多样性分析曾淇.pdf

2010, 11( 3): 298-304 Journal of P lant Genetic Resources 基于 SSR标记的荔枝种质遗传多样性分析 1, 2 1 1 曾 淇 , 李明芳 , 郑学勤 1 2 ( , 571101; , 571101) : 从 53对本课题组自主开发设计的 R特异引物对中筛选出 22 对多态 引物, 对 46份荔枝材料的基因组 DNA 进 行扩增, 共得到 54 个等位基因, 其中每对引物的平均等位基因数为 214, 共获得 23 个特异 标记, 各标记的平均观察杂合度 值平均期望杂合度和PIC 值分别为 0145101355和 01 507其中Lit6位点各数值最高, 是最理想的选择标记利用 22 对多 态 引物对 46份荔枝种质进行聚类分析, 构建树状图, 结果表明: 大多数种质相似系数在 01 63~ 01 95之间, 说明它们之间的 亲缘关系较近, 并发现淮枝(广东)与淮枝(海南) 两者可能为同名异物品种 : 荔枝; R标记; 遗传多样 AnalysisofGeneticDiversitywithinLitchiVarieties based on SSRMarkers 1, 2 1 1 ZENG Q i , LIM ing- fang , ZHENG Xue- qin 1 ( Institute of Trop ical B ioscience and B io techno logy, Ch ineseA cademy of Trop icalA gricultural Sciences, 2 H a ikou 571101; H ainan Uni ersity of A griculture, H aikou 571101) Abstract:Tw enty-wt o polymorphic prmi er pairsw ere selected from 53 R special prmi er pairs developed and designed by our laboratory to amplify genom ic DNA of 46 litch.i 23 locus-specific R markersw ere obtained, and 54 allelesw ere found and the average alleles per marker w as 2. 4. The average observed heterozygosity, expected heterozygosity andPIC of allmarkersw ere 0. 451, 0. 355 and 0. 507 respectively. Locus Lit6 had the highest num- ber show ed that itw as an optmi almarker. Then the selected polymorph ic R m arkersw ere used to detect the ge- netic d iversity of 46 litch i germplasm and to cluster them. The resu lts revealed that smi ilarity coefficients ofmost of litchi germplasm w ere among