芹菜素对慢性间歇低氧幼鼠认知损害的保护作用.docVIP

芹菜素对慢性间歇低氧幼鼠认知损害的保护作用 　　作者：张存雪 作者单位：温州医学院附属育英儿童医院 呼吸科，浙江 温州 325027 　　【摘要】 目的:研究芹菜素对慢性间歇低氧幼鼠认知损害的保护作用。方法:八臂迷宫训练成功的3～4周龄雄性SD幼鼠40只，随机分空气对照组(C组)、间歇低氧组(H组)、间歇低氧二甲基亚砜组(R组)、间歇低氧芹菜素+二甲基亚砜组(Q组)。间歇低氧2周后八臂迷宫测试全部幼鼠学习记忆能力。以透射电镜观察海马CA1区神经元的超微结构变化。结果:与R组比，Q组参考记忆错误(RME)和总记忆错误(TE)显著降低(P0.05，P0.01)。与C组比，H组RME、工作记忆错误(WME)和TE显著增高(P0.01，P0.05，P0.01)。电镜下H组和R组海马CA1区神经细胞的形态、胞浆内的细胞器与正常细胞比较有明显变化，Q组和C组无明显变化。结论:芹菜素对于慢性间歇低氧幼鼠海马神经元具有保护作用，从而减轻慢性间歇低氧对幼鼠空间学习记忆能力的损伤。 　　【关键词】 慢性间歇低氧 芹菜素 记忆 海马 超微结构 　　Protective function of apigenin on the damage of congnition in immatcore rats with chronic intermittent hypoxia. ZHANG Cun-xue, CAI Xiao-hong, CAO Shu-yan，ZHOU Yong-hai. Department of Respiratory Medicine，Yuying Childrens Hospital Affiliated to Wenzhou Medical College, Wenzhou, 325027. 　　Abstract: Objective: To explore the protective effect of apigenin in immature rats treated with chronic intermittent hypoxia. Methods: Forty 3～4 week-old SD rats which have been trained to complete the 8-Arm (4-arm baited) radial maze were randomly divided into air control(C) group, intermittent hypoxia(H) group, DMSO control(R) group and apigenin + DMSO(Q) group. After 2 weeks intermittent hypoxia, these immature rats were tested by 8-Arm (4-arm baited) radial maze to measure spatial memory and the ultrastructure of hippocampal CAl neurons was studied under electron microscope. Results: Compared with R group, Q group had significantly less in reference memory error and total memory error (P0.05, P0.01).Compared with C group, H group had significantly reference，working and total memory error, respectively(P0.01, P0.05, P0.01).The nuclear membrane of hippocampal CAl neurons in H group and R group were distorted compared with normal cells; Q group and C group had no changes. Conclusion: Apigenin protects hippocampal neuron in immature rats, and improves spatial learning and memory damaged by chronic intermittent hypoxia. 　　Key words: chronic intermittent hypoxia; apigenin; memory; hippocampus; ultrastru