一个新的水稻短根毛突变体的遗传分析和基因定位.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2012, 38(2): 240244 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2012.00240 一个新的水稻短根毛突变体的遗传分析和基因定位 丁沃娜1 黄 炜2 宁永强1 朱世华1 1 315211; 2 310058 : T-DNA 11 , ossrh3 (Oryza sativa short root hair 3), 1 , ossrh3 Kasalath F2 , SSR (simple sequence repeat) STS (sequence- tagged site), OsSRH3 1 S38978 S39016 , 37.7 kb, 8 , OsSRH3 : (Oryza sativa L.); ; ; Genetic Analysis and Gene Mapping of a Novel Short Root Hair Mutant in Rice 1 2 1 1 DING Wo-Na , HUANG Wei , NING Yong-Qiang , and ZHU Shi-Hua 1 Laboratory of Plant Molecular Biology, Ningbo University, Ningbo 315211, China; 2 State Key Laboratory of Plant Physiology and Biochemistry, Zhejiang University, Hangzhou 310058, China Abstract: In plants, root hair is important organ for the uptake of nutrients and water. A rice (Oryza sativa L.) mutant with short root hairs (ossrh3) was isolated from a T-DNA insertion mutant library with Zhonghua 11 background. The elongation of root hairs in the mutant was severely impaired, and other traits were also affected including the plant height, primary root length, lat- eral root length, and number of lateral roots. Genetic analysis indicated that the mutant phenotype was controlled by a single re- cessive nuclear gene. For mapping the OsSRH3 gene, an F population was generated by crossing the mutant ossrh3 with Kasalath 2 wild type. Ultimately by using the published SSR (simple sequence repeat) markers and some newly designed STS (se- quence-tagged site) markers, the OsSRH3 locus was mapped to a 37.7 kb region between the