胶原基纳米骨的遗传毒性和对体外培养细胞影响的研究.docVIP

胶原基纳米骨的遗传毒性及对体外培养细胞影响的研究 胶原基纳米骨的遗传毒性及对体外培养细胞影响的研究 作者：陈鹏　毛天球　刘冰　岳进　雷德林　杨耀武 　　［摘要］ 目的：体外研究胶原基纳米骨的遗传毒性，及对原代培养的人牙周膜成纤维样细胞、兔成骨细胞的影响。方法：采用Ames致突变试验、MTT法及碱性磷酸酶（ALP）检测法，测定不同浓度胶原基纳米骨(nHAC)的浸提液对鼠伤寒沙门菌的致突变比值的影响和对人牙周膜成纤维样细胞及兔成骨细胞的影响。结果：nHAC的浸提液各剂量组对鼠伤寒沙门菌的致突变比值均小于2，nHAC不会引起鼠伤寒沙门菌的回复突变数增加。不同时间点用不同浓度浸提液培养的人牙周膜成纤维样细胞正常增殖，浸提液不影响兔成骨细胞的功能表达。结论：胶原基纳米骨无遗传毒性，不影响人牙周膜成纤维样细胞的增殖活性和兔成骨细胞的成骨活性，是一种组织工程骨支架的良好材料。 　　［关键词］ 纳米 生物材料 胶原基纳米骨 遗传毒性 　　Genetoxicity of Collagen Based Composite and Its Influence on Cultured Cell in Vitro. CHEN Peng, MAO Tian-qiu, LIU Bing, et al. Stomatlogical College, Fourth Military Medical University, Xian 710032 　　［Abstract］Objective: To study the genetoxicity of nano-hydroxyapatite/collogen (nHAC) in vitro and the influence on primary cultured human periodontal ligament fibroblast-like cells(PDLF) and rabbit osteoblasts by nHAC. Methods: The Ames test, MTT assay and alkaline phosphatase(ALP) activity test were carried out to evaluate the influence on the mutagenesis rates of murine typhoid salmonella, on the PDLF and rabbit osteoblasts by different density nHAC leaching liquid. Results: The mutagenesis rates of murine typhoid salmonella in the experimental groups of all dosage levers were less then 2, and nHAC would not cause the increase of the back mutation in murine typhoid salmonella. The PDLF proliferation rate was normal in the infusion compared to standard DMEM culture medium. Infusion had no effects on the functional expression of rabbit osteoblasts. Conclution: nHAC has not the inherited toxicity, and induces no cytotoxicity effect on the activity of PDLF and osteoblasts. It can be used as a tissue engineering scaffold. 　　［Key words］ Nanometer Biomaterials Collagen based composite Genetoxic 近年来利用各种材料的优缺点将多个材料进行复合，制作具有更好的生物兼容性、骨引导性、可吸收性及强度的支架材料是骨组织工程的研究热点之一。清华大学材料科学与工程系必威体育精装版研制的多孔块状胶原基纳米骨（nano-hydroxyapatite/collogen，nHAC）是目前各项性能较好的复合材料之一［1］，本实验试图进一步研究nHAC的遗传学毒性及对人牙周膜成纤维样细胞、兔成骨细胞的影响，为其在口腔颌面外科的临床应用提供实验基础。 1 材料与方法