青花菜高效离体再生体系的建立.pdfVIP

第 27 卷第 5期 西 南 农 业 大 学 学 报 ( 自然科学版 ) V ol. 27, No. 5 2005年 10 月 Journal of Southw estAgriculturalUniversit (N atural Science) Oct. 2005 : 1000- 2642( 2005) 05- 0653- 04 1 2* 2 1 2 秦耀国, 雷建军 , 曹必好 , 李焕秀 , 谢文华 ( 1. , 625014; 2. , 5 10642) : 以青花菜为试验材料, 探讨了影响离体 生的一些因素, 结果表明: 取用 4~ 5 d龄无菌苗的带柄子叶和下胚轴, 分别培养在M SB+ NAA 0. 2 m g/L+ 6- BA 2 m g/L和MSB + NAA 0. 2 m g/L+ 6- BA 1. 0 mg /L+ AgNO 2. 5 m g/L培养 3 基上, 获得了较高的分化率( 80% ); 生芽插入M S+ IBA 0. 2 mg /L培养基, 生根率达 100% : 青花菜; 生; 分化; AgNO3 : S 635. 901 : A ESTABLISHMENT OF A H IGHLY EFFICIENT IN VITRO REGENERAT ION SYSTEM FOR BROCCOL I 1 2* 2 1 2 QIN Yao- guo, LEI Jian- jun , CAO Bi- hao, LIHuan- xiu, XIEW en- hua ( 1. College of Forestr and H orticulture, S ichuan Agricultural Un iversit , Y aan, S ichuan 6250 14, Ch ina; 2. College of H orticulture, Sou th Ch inaAgricu ltu ralUn iversit , Guangzhou, Guangdong 5 10642, Ch ina) Abstract: Cot ledons w ith petiole and h pocot ls of 4- to 5- da - old virus- free seed lings of broccoli (B rassica oleracea var. italica ) w ere used as explants in in v itro cu lture. Fairl h igh shoot differentiation rate ( gt; 80% ) w as ob tained when cot ledons w ere cultured on M SB + NAA 0. 2 mg /L + 6- BA 2 mg /L and h pocot ls were cu ltured on M SB + NAA 0. 2 m g/L + 6 - BA 1. 0 mg /L + AgNO3 2. 5 m g/L. A ll the shoots rooted read il when transferred toM S + IBA 0. 2 mg/L. ey words: broccoli; regeneration; differentiation; AgNO3 (B rass ica oleracea L. var. italica) , 1, a ; , , , 20 70 ,