秋水仙素对大蒜生长的影响及多倍体诱导效应分析.pdfVIP

, Jour al of A hui Agri. Sci. 2009, 37(9) : 4191- 4194 王淼 况玲玲 * 谢晓玲, 邓自发 ( , 226007) [ 目的] 探讨诱导大蒜染色体加倍的有效方法[ 方法] 以秋水仙素为诱变剂, 较不同浓度和不同时间处理对大蒜的多倍体诱 导效应[ 结果] 经秋水仙素处理过的植株, 在相同浓度下, 随着秋水仙素处理时间的延长, 株高降低, 细胞有丝分裂指数减小, 染色体加 倍率升高在相同的处理时间内, 随着秋水仙素浓度的升高, 株高降低, 细胞有丝分裂指数明显减小, 染色体加倍率显著升高当秋水 仙素浓度为0. 05%, 处理6 d 时, 染色体加倍率达到最高, 为28. 67% 随着浓度的增加和处理时间的延长, 植株的死亡率逐渐上升, 气孔 面积也呈上升趋势[ 结论] 该研究为丰富大蒜种质资源和加速高产优质大蒜的育种进程奠定了基础 大蒜; 多倍体; 秋水仙素; 诱导效应 S 633.4 A 0517- 6611( 2009) 09- 04191- 04 Analysis on the Influences of Colchicine on the Growth of Allium sati vum and Its Polyploid Induction Effect XIE Xiao-ling et al ( School of Life Scie ces, Na to g U iversity, Na to g, Jia gsu 226007) Abstract [ Objective] The aim was to discuss the effectivemethod for i duci g A llium sativum polyploid doubled. [Method] With colchici e as muta- ge , the i ductive effects of colchici e with differe t co c . o A . sativum polyploid i differe t treatme t time were compared. [ Result] Whe the pla ts were treated by colchici e, the pla t height was decreased, the cell mitotic i dex was decreased a d the chromosome-doubled rate was i creased with the prolo gi g of treatme t timeof colchici e i the same co c . The pla t height was decreased, the cell mitotic i dexwas obviously decreased a d the chromosome-doubled rate was sig ifica tly i creased with the i creasi g of colchici e co c . i the same treatme t time. Whe the colchici e co c . was 0. 05% a d the treatme t time was 6 d, the chromosome-doubled ratewas highest, bei g 28.67%. The pla t mortality was i creased gradually a d the stomatal size was also i creased with the i creasi g of co c . a d prolo gi g of treatme t time. [ Co clusio ] Theresearch laid the fou datio for the abu da ce of A . sativum germplasm resource a d acceleratio of the breedi g process of high yield a d quality A . sativum. Key words A llium sativum; Pol