单细胞测序_技术_应用和未来发展_英文_.pdfVIP

Sci. Bull. (2015) 60(1):33–42 DOI 10.1007/s11434-014-0634-6 Review Single cell sequencing: technique, application, and future development Ruidong Xue • Ruoyan Li • Fan Bai Received: 3 July 2014 / Accepted: 8 August 2014 / Published online: 17 December 2014 Science China Press and Springer-Verlag Berlin Heidelberg 2014 Abstract The progression of next generation sequencing Many biological experiments and assays are performed on is continuously changing the landscape of genomic, tran- cells in bulk, assuming that all cells of a particular ‘‘type’’ scriptomic, and epigenomic studies. Particularly, advances are identical. However, recent evidence from single cell in single cell manipulation and amplification techniques studies reveals that this assumption is not completely true bring sequencing technology to the single-cell level. Single [1, 2]. Individual cells within the same population may, on cell genome sequencing allows us to study tumor evolu- various aspects, differ dramatically, thus making different tion, gamete genesis, somatic mosaicism at genome-wide contributions to the health and function of the entire pop- level; single cell transcriptome sequencing unveils the ulation. Experimental approaches that only measure the dynamic gene expression during early embryonic devel- population-level characteristics may average or dilute the opment, differentiation and reprogramming; single cell vital differences among cells. For instance, the heteroge- methylome sequencing is just taking off and shows great neous nature of cancer cells makes it difficult and inaccu- potential in cancer and stem cell studies. Lots of attempts rate, by bulk sequencing, to asse